ArticleNature chemistry2013
A clamp-like biohybrid catalyst for DNA oxidation.
Article in Nature chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 72 citations in OpenAlex.
- Tangled Tail of Mechanically Interlocked Peptides.Journal of the American Chemical Society · 2026Review
- Click and Cut: a click chemistry approach to developing oxidative DNA damaging agents.Nucleic acids research · 2021Article
- Ru(II)Porphyrinate-based molecular nanoreactor for carbene insertion reactions and quantitative formation of rotaxanes by active-metal-template syntheses.Nature communications · 2020Article
- AT-CuAAC Synthesis of Mechanically Interlocked Oligonucleotides.Journal of the American Chemical Society · 2020Article
- Catalytic transport of molecular cargo using diffusive binding along a polymer track.Nature chemistry · 2019Article
- A distributive peptide cyclase processes multiple microviridin core peptides within a single polypeptide substrate.Nature communications · 2018Article
- Synthetic polymers as substrates for a DNA-sliding clamp protein.Biopolymers · 2018Article
- An artificial molecular machine that builds an asymmetric catalyst.Nature nanotechnology · 2018Article
- Repeat protein scaffolds: ordering photo- and electroactive molecules in solution and solid state.Chemical science · 2016Article
- High yielding synthesis of 2,2'-bipyridine macrocycles, versatile intermediates in the synthesis of rotaxanes.Chemical science · 2016Article
- Asymmetric Catalysis with a Mechanically Point-Chiral Rotaxane.Journal of the American Chemical Society · 2016Article
- A Stimuli-Responsive Rotaxane-Gold Catalyst: Regulation of Activity and Diastereoselectivity.Angewandte Chemie (International ed. in English) · 2015Article
- Molecular computing: paths to chemical Turing machines.Chemical science · 2015Article
- Reversible mechanical switching of magnetic interactions in a molecular shuttle.ChemistryOpen · 2015Article
- Processive catalysis: Thread and cut.Nature chemistry · 2013Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
In processive catalysis, a catalyst binds to a substrate and remains bound as it performs several consecutive reactions, as exemplified by DNA polymerases. Processivity is essential in nature and is often mediated by a clamp-like structure that physically tethers the catalyst to its (polymeric) template. In the case of the bacteriophage T4 replisome, a dedicated clamp protein acts as a processivity mediator by encircling DNA and subsequently recruiting its polymerase. Here we use this DNA-binding protein to construct a biohybrid catalyst. Conjugation of the clamp protein to a chemical catalyst with sequence-specific oxidation behaviour formed a catalytic clamp that can be loaded onto a DNA plasmid. The catalytic activity of the biohybrid catalyst was visualized using a procedure based on an atomic force microscopy method that detects and spatially locates oxidized sites in DNA. Varying the experimental conditions enabled switching between processive and distributive catalysis and influencing the sliding direction of this rotaxane-like catalyst.
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