ArticleJournal of clinical microbiology2014
Optimal culture incubation time in orthopedic device-associated infections: a retrospective analysis of prolonged 14-day incubation.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 3 of them syntheses that pooled it.
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Who cites it
33 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Optimized use and performance of culture for periprosthetic joint infection diagnosis: a comprehensive literature review.Clinical microbiology reviews · 2025Pooled it
- Surprise positive culture rate in the treatment of presumed aseptic long-bone nonunion: a systematic review with meta-analysis of 2397 patients.Archives of orthopaedic and trauma surgery · 2024Pooled it
- Meta-analysis of sonication fluid samples from prosthetic components for diagnosis of infection after total joint arthroplasty.Journal of clinical microbiology · 2014Pooled it
- How Does the Time from Intraoperative Culture Harvest to Incubation Impact Culture Positivity in Orthopaedic Surgeries? A Case-Control Study.Antibiotics (Basel, Switzerland) · 2026Article
- Currently applied sonication protocols for the detection of implant-associated infections in orthopaedics: a scoping review.Journal of clinical microbiology · 2026Article
- Data from the Swiss National Arthroplasty Registry SIRIS suggest that unicompartmental knee arthroplasty is associated with a lower risk of periprosthetic joint infection than total knee arthroplasty.Archives of orthopaedic and trauma surgery · 2026Article
- Gentamicin fails to eradicateJournal of bone and joint infection · 2026Article
- Microbiology, risk factors, and clinical outcomes of low-grade infection in long-bone diaphyseal non-union after open fracture.International orthopaedics · 2026Article
- Fracture nonunions.OTA international : the open access journal of orthopaedic trauma · 2025Article
- Extending the culture duration could not improve the culture positivity rate and clinical outcomes of periprosthetic joint infection.Frontiers in cellular and infection microbiology · 2025Article
- Systemic antibiotic prophylaxis in arthroplasty - a narrative review of how many doses are optimal.EFORT open reviews · 2024Review
- Antibiotic utilisation in primary and revision total hip replacement patients: A registry linkage cohort study of 106 253 patients using the Australian Orthopaedic Association National Joint Replacement Registry.Pharmacoepidemiology and drug safety · 2023Article
- Molecular Characterization of Community- and Hospital- Acquired Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Recurrent Failures After 2-Stage Exchanges are Secondary to New Organisms Not Previously Covered by Antibiotics.Arthroplasty today · 2022Article
- Enhancing Diagnostics in Orthopedic Infections.Journal of clinical microbiology · 2022Review
- Positivity Trends of Bacterial Cultures from Cases of Acute and Chronic Periprosthetic Joint Infections.Journal of clinical medicine · 2022Article
- A Prospective Study to Assess the Optimal Incubation Times for Culture and Aerobic Bacterial Profile in Prosthetic Joint Infections.Journal of laboratory physicians · 2021Article
- Influence of ceftriaxone on human bone cell viability and in vitro mineralization potential is concentration- and time-dependent.Bone & joint research · 2021Article
- Hip aspiration culture: analysing data from a single operator series investigating periprosthetic joint infection.Journal of bone and joint infection · 2021Article
- Epidemiology, resistance characteristics, virulence determinants, and treatment outcomes ofPathogens and global health · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate diagnosis of orthopedic device-associated infections can be challenging. Culture of tissue biopsy specimens is often considered the gold standard; however, there is currently no consensus on the ideal incubation time for specimens. The aim of our study was to assess the yield of a 14-day incubation protocol for tissue biopsy specimens from revision surgery (joint replacements and internal fixation devices) in a general orthopedic and trauma surgery setting. Medical records were reviewed retrospectively in order to identify cases of infection according to predefined diagnostic criteria. From August 2009 to March 2012, 499 tissue biopsy specimens were sampled from 117 cases. In 70 cases (59.8%), at least one sample showed microbiological growth. Among them, 58 cases (82.9%) were considered infections and 12 cases (17.1%) were classified as contaminations. The median time to positivity in the cases of infection was 1 day (range, 1 to 10 days), compared to 6 days (range, 1 to 11 days) in the cases of contamination (P < 0.001). Fifty-six (96.6%) of the infection cases were diagnosed within 7 days of incubation. In conclusion, the results of our study show that the incubation of tissue biopsy specimens beyond 7 days is not productive in a general orthopedic and trauma surgery setting. Prolonged 14-day incubation might be of interest in particular situations, however, in which the prevalence of slow-growing microorganisms and anaerobes is higher.
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