ArticleOpen biology2013
Pleiotropic regulatory genes bldA, adpA and absB are implicated in production of phosphoglycolipid antibiotic moenomycin.
Article in Open biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 68 citations in OpenAlex.
- The Potential of Non-Ribosomal Peptide Engineering for Creating New Antimicrobial Complexes.Molecules (Basel, Switzerland) · 2026Review
- Probing the function ofMicrobiology spectrum · 2026Article
- AdpA, a Global Regulator of Hundreds of Genes, Including Those for Secondary Metabolism, inAntibiotics (Basel, Switzerland) · 2025Article
- Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5'-phosphate (vitamin B6) in Streptomyces coelicolor M145.BMC microbiology · 2025Article
- Regulatory Genes as Beacons for Discovery and Prioritization of Biosynthetic Gene Clusters inBiochemistry · 2025Article
- Global regulator AdpA directly binds to tunicamycin gene cluster and negatively regulates tunicamycin biosynthesis in Streptomyces clavuligerus.World journal of microbiology & biotechnology · 2024Article
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- Designing of Multi-Epitope Peptide Vaccine Based on Outer Membrane Proteins OmpF, OmpC, and PgtE ofArchives of Razi Institute · 2023Article
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- Flavacol and Its Novel Derivative 3-Microorganisms · 2022Article
- Global Regulator AdpA_1075 Regulates Morphological Differentiation and Ansamitocin Production inBioengineering (Basel, Switzerland) · 2022Article
- Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR-Cas9.Bioresources and bioprocessing · 2022Article
- AdpA, a developmental regulator, promotes ε-poly-L-lysine biosynthesis in Streptomyces albulus.Microbial cell factories · 2022Article
- Genetic approaches to improve clorobiocin production in Streptomyces roseochromogenes NRRL 3504.Applied microbiology and biotechnology · 2022Article
- Computational Design of a Multi-Epitope Vaccine AgainstFrontiers in immunology · 2022Article
- Structural diversity, bioactivity, and biosynthesis of phosphoglycolipid family antibiotics: Recent advances.BBA advances · 2022Article
- Genetic Engineering ofMicroorganisms · 2021Article
- Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces.World journal of microbiology & biotechnology · 2021Article
- The Use of the Rare TTA Codon inIndian journal of microbiology · 2021Article
- Eliciting the silent lucensomycin biosynthetic pathway in Streptomyces cyanogenus S136 via manipulation of the global regulatory gene adpA.Scientific reports · 2021Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Unlike the majority of actinomycete secondary metabolic pathways, the biosynthesis of peptidoglycan glycosyltransferase inhibitor moenomycin in Streptomyces ghanaensis does not involve any cluster-situated regulators (CSRs). This raises questions about the regulatory signals that initiate and sustain moenomycin production. We now show that three pleiotropic regulatory genes for Streptomyces morphogenesis and antibiotic production-bldA, adpA and absB-exert multi-layered control over moenomycin biosynthesis in native and heterologous producers. The bldA gene for tRNA(Leu)UAA is required for the translation of rare UUA codons within two key moenomycin biosynthetic genes (moe), moeO5 and moeE5. It also indirectly influences moenomycin production by controlling the translation of the UUA-containing adpA and, probably, other as-yet-unknown repressor gene(s). AdpA binds key moe promoters and activates them. Furthermore, AdpA interacts with the bldA promoter, thus impacting translation of bldA-dependent mRNAs-that of adpA and several moe genes. Both adpA expression and moenomycin production are increased in an absB-deficient background, most probably because AbsB normally limits adpA mRNA abundance through ribonucleolytic cleavage. Our work highlights an underappreciated strategy for secondary metabolism regulation, in which the interaction between structural genes and pleiotropic regulators is not mediated by CSRs. This strategy might be relevant for a growing number of CSR-free gene clusters unearthed during actinomycete genome mining.
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