ArticleRNA (New York, N.Y.)2013
Structural and functional analysis of Utp23, a yeast ribosome synthesis factor with degenerate PIN domain.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation.Nature communications · 2025Article
- Synergistic interaction network between the snR30 RNP, Utp23, and ribosomal RNA during ribosome synthesis.RNA biology · 2022Article
- snR30/U17 Small Nucleolar Ribonucleoprotein: A Critical Player during Ribosome Biogenesis.Cells · 2020Review
- Mapping of developmental dysplasia of the hip to two novel regions at 8q23-q24 and 12p12.Experimental and therapeutic medicine · 2020Article
- Article
- Conformational switches control early maturation of the eukaryotic small ribosomal subunit.eLife · 2019Article
- Structural and functional analysis of Utp24, an endonuclease for processing 18S ribosomal RNA.PloS one · 2018Article
- 3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.Nature structural & molecular biology · 2017Article
- Structural conservation of the PIN domain active site across all domains of life.Protein science : a publication of the Protein Society · 2017Review
- Comprehensive classification of the PIN domain-like superfamily.Nucleic acids research · 2017Article
- The ribosome biogenesis factor yUtp23/hUTP23 coordinates key interactions in the yeast and human pre-40S particle and hUTP23 contains an essential PIN domain.Nucleic acids research · 2017Article
- Stepwise and dynamic assembly of the earliest precursors of small ribosomal subunits in yeast.Genes & development · 2016Article
- Association of 8q23-24 region (8q23.3 loci and 8q24.21 loci) with susceptibility to colorectal cancer: a systematic and updated meta-analysis.International journal of clinical and experimental medicine · 2015Article
- Interaction between ribosome assembly factors Krr1 and Faf1 is essential for formation of small ribosomal subunit in yeast.The Journal of biological chemistry · 2014Article
- Structure of Utp21 tandem WD domain provides insight into the organization of the UTPB complex involved in ribosome synthesis.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During synthesis of yeast ribosome, a large complex, called the 90S pre-ribosome or the small subunit processome, is assembled on the nascent precursor rRNA and mediates early processing of 18S rRNA. The Utp23 protein and snR30 H/ACA snoRNA are two conserved components of 90S pre-ribosomes. Utp23 contains a degenerate PIN nuclease domain followed by a long C-terminal tail and associates specifically with snR30. Here, we report the crystal structure of the Utp23 PIN domain at 2.5-Å resolution. The structure reveals a conserved core fold of PIN domain with degenerate active site residues, a unique CCHC Zn-finger motif, and two terminal extension elements. Functional sites of Utp23 have been examined with conservation analysis, mutagenesis, and in vivo and in vitro assays. Mutations in each of three cysteine ligands of zinc, although not the histidine ligand, were lethal or strongly inhibitory to yeast growth, indicating that the Zn-finger motif is required for Utp23 structure or function. The N-terminal helix extension harbors many highly conserved basic residues that mostly are critical for growth and in vitro RNA-binding activity of Utp23. Deletion of the C-terminal tail, which contains a short functionally important sequence motif, disrupted the interaction of Utp23 with snR30 and perturbed the pre-ribosomal association of Utp23. Our data establish a structural framework for dissecting Utp23 function in the assembly and dynamics of 90S pre-ribosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.