ArticleJournal of the American Chemical Society2013
Translocation of cationic amphipathic peptides across the membranes of pure phospholipid giant vesicles.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Membrane-Traversing Peptides: Mechanistic Landscapes, Assays, and Applications.Chemical reviews · 2026Review
- Dual-Channel Fluorescence Assays with Supramolecular Host-Dye Reporter Pairs for Membrane Activity Mapping of Peptides.Angewandte Chemie (International ed. in English) · 2026Article
- Passage of the Channel-Forming Agent Nystatin Through Ergosterol-Containing Lipid Membranes.The Journal of membrane biology · 2025Article
- A unified model of transient poration induced by antimicrobial peptides.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Membrane Activity of Melittin and Magainin-I at Low Peptide-to-Lipid Ratio: Different Types of Pores and Translocation Mechanisms.Biomolecules · 2024Article
- Melittin can permeabilize membranes via large transient pores.Nature communications · 2024Article
- Peptide translocation across asymmetric phospholipid membranes.Biophysical journal · 2024Article
- A molecular dynamics study of cell-penetrating peptide transportan-10 (TP10): Binding, folding and insertion to transmembrane state in zwitterionic membrane.Biochimica et biophysica acta. Biomembranes · 2024Article
- Electrochemiluminescence Imaging of Liposome Permeabilization by an Antimicrobial Peptide: Melittin.Chemical & biomedical imaging · 2023Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
- The Antibiotic Peptide Daptomycin Functions by Reorganizing the Membrane.The Journal of membrane biology · 2021Review
- Effect of Vesicle Size on the Cytolysis of Cell-Penetrating Peptides (CPPs).International journal of molecular sciences · 2020Article
- Key Process and Factors Controlling the Direct Translocation of Cell-Penetrating Peptide through Bio-Membrane.International journal of molecular sciences · 2020Article
- Supramolecular Chemistry in the Biomembrane.Chembiochem : a European journal of chemical biology · 2020Review
- Revisiting the Interaction of Melittin with Phospholipid Bilayers: The Effects of Concentration and Ionic Strength.International journal of molecular sciences · 2020Article
- Spectroscopic and Microscopic Approaches for Investigating the Dynamic Interactions of Anti-microbial Peptides With Membranes and Cells.Frontiers in medical technology · 2020Review
- Using fluorescence microscopy to shed light on the mechanisms of antimicrobial peptides.Future medicinal chemistry · 2019Review
- Mechanistic Landscape of Membrane-Permeabilizing Peptides.Chemical reviews · 2019Review
- Trapped! A Critical Evaluation of Methods for Measuring Total Cellular Uptake versus Cytosolic Localization.Bioconjugate chemistry · 2019Review
- HaloTag Assay Suggests Common Mechanism of E. coli Membrane Permeabilization Induced by Cationic Peptides.ACS chemical biology · 2018Article
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Authors and funding
5 authors.
Funding
Abstract
The ability of amphipathic polypeptides with substantial net positive charges to translocate across lipid membranes is a fundamental problem in physical biochemistry. These peptides should not passively cross the bilayer nonpolar region, but they do. Here we present a method to measure peptide translocation and test it on three representative membrane-active peptides. In samples of giant unilamellar vesicles (GUVs) prepared by electroformation, some GUVs enclose inner vesicles. When these GUVs are added to a peptide solution containing a membrane-impermeant fluorescent dye (carboxyfluorescein), the peptide permeabilizes the outer membrane, and dye enters the outer GUV, which then exhibits green fluorescence. The inner vesicles remain dark if the peptide does not cross the outer membrane. However, if the peptide translocates, it permeabilizes the inner vesicles as well, which then show fluorescence. We also measure translocation, simultaneously on the same GUV, by the appearance of fluorescently labeled peptides on the inner vesicle membranes. All three peptides examined are able to translocate, but to different extents. Peptides with smaller Gibbs energies of insertion into the membrane translocate more easily. Further, translocation and influx occur broadly over the same period, but with very different kinetics. Translocation across the outer membrane follows approximately an exponential rise, with a characteristic time of 10 min. Influx occurs more abruptly. In the outer vesicle, influx happens before most of the translocation. However, some peptides cross the membrane before any influx is observed. In the inner vesicles, influx occurs abruptly sometime during peptide translocation across the membrane of the outer vesicle.
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