ArticleCombinatorial chemistry & high throughput screening2014
Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening.
Article in Combinatorial chemistry & high throughput screening, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 102 citations in OpenAlex.
- Rapid Diagnostics for Distinguishing Bacterial and Viral Infections: A Review of Technologies, Clinical Utility, and Stewardship Implications.Biosensors · 2026Review
- Digital Holographic Microscopy for Phenotypic Profiling of Adherent Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Dissecting Morphological and Functional Dynamics of Non-Tumorigenic and Triple-Negative Breast Cancer Cell Lines Using PCA and t-SNE Analysis.Cancer reports (Hoboken, N.J.) · 2025Article
- Holographic Proteomics: A Review of Digital Holographic Microscopy Applications in Spatial Proteomics.Protein and peptide letters · 2025Review
- Advances in Microscopy and Its Applications with Special Reference to Fluorescence Microscope: An Overview.Advances in experimental medicine and biology · 2023Article
- Label-free viability assay using in-line holographic video microscopy.Scientific reports · 2022Article
- Nanospermidine in Combination with Nanofenretinide Induces Cell Death in Neuroblastoma Cell Lines.Pharmaceutics · 2022Article
- Characterization and identification of cell death dynamics by quantitative phase imaging.Journal of biomedical optics · 2022Article
- Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules.International journal of molecular sciences · 2021Article
- High-throughput label-free cell detection and counting from diffraction patterns with deep fully convolutional neural networks.Journal of biomedical optics · 2021Article
- Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy.Scientific reports · 2021Article
- Root Extracts of Two Cultivars ofMolecules (Basel, Switzerland) · 2021Article
- Acoustofluidic phase microscopy in a tilted segmentation-free configuration.Biomicrofluidics · 2021Article
- Article
- Morphology, Motility, and Cytoskeletal Architecture of Breast Cancer Cells Depend on Keratin 19 and Substrate.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays.Scientific reports · 2020Article
- Image-Based Marker-Free Screening of GABASLAS discovery : advancing life sciences R & D · 2020Article
- Restoration of Physiological Levels of Uric Acid and Ascorbic Acid Reroutes the Metabolism of Stored Red Blood Cells.Metabolites · 2020Article
- Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods.Optics express · 2020Article
- Quantitative scoring of epithelial and mesenchymal qualities of cancer cells using machine learning and quantitative phase imaging.Journal of biomedical optics · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractive index and to cell thickness. DHM is able to distinguish cellular morphological changes on two representative cell lines (HeLa and H9c2) when treated with doxorubicin and chloroquine, two cytotoxic compounds yielding distinct phenotypes. We analyzed parameters linked to cell morphology and to the intracellular content in endpoint measurements and further investigated them with timelapse recording. The results obtained by DHM were compared with other optical label-free microscopy techniques, namely Phase Contrast, Differential Interference Contrast and Transport of Intensity Equation (reconstructed from three bright-field images). For comparative purposes, images were acquired in a common 96-well plate format on the different motorized microscopes. In contrast to the other microscopies assayed, images generated with DHM can be easily quantified using a simple automatized on-the-fly analysis method for discriminating the different phenotypes generated in each cell line. The DHM technology is suitable for the development of robust and unbiased image-based assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.