Evidence map›Paper›PMID 24152191›Full record

ArticleJournal of the American Chemical Society2013

Chemoproteomic discovery of cysteine-containing human short open reading frames.

Adam G Schwaid, D Alexander Shannon, Jiao Ma, Sarah A Slavoff, Joshua Z Levin, Eranthie Weerapana, Alan Saghatelian

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

7 authors.

Adam G Schwaid *Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
D Alexander Shannon *Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA.
Jiao MaDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Sarah A SlavoffDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Joshua Z LevinGenome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Eranthie WeerapanaDepartment of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA.
Alan SaghatelianDepartment of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.

Funding

Large Scale Sequencing and Analysis of GenomesU54HG003067 · NHGRI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GABRIEL, STACEY, LANDER, ERIC S · 2004 to 2015
$568.6M
The Discovery of Human Peptide Encoding GenesR01GM102491 · NIGMS · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI O'SHEA, CLODAGH, SAGHATELIAN, ALAN · 2012 to 2025
$5.5M
Scope and mechanism of eptide translation from short open reading frames in the hF32GM099408 · NIGMS · HARVARD UNIVERSITY · PI SLAVOFF, SARAH ANN · 2011 to 2013
$147k
NHGRI NIH HHS HG03067NHGRI NIH HHS U54 HG003067NIGMS NIH HHS 1F32GM099408-01NIGMS NIH HHS F32 GM099408NIGMS NIH HHS R01 GM102491NIGMS NIH HHS R01GM102491
6 · The paper itself

Abstract

The application of ribosome profiling and mass spectrometry technologies has recently revealed that the human proteome is larger than previously appreciated. Short open reading frames (sORFs), which are difficult to identify using traditional gene-finding algorithms, constitute a significant fraction of unknown protein-coding genes. Thus, experimental approaches to identify sORFs provide invaluable insight into the protein-coding potential of genomes. Here, we report an affinity-based approach to enrich and identify cysteine-containing human sORF-encoded polypeptides (ccSEPs) from cells. This approach revealed 16 novel ccSEPs, each derived from an uncharacterized sORF, demonstrating its potential for discovering new genes. We validated expression of a SEP from its endogenous RNA, and demonstrated the specificity of our labeling approach using synthetic SEP. The discovery of additional human SEPs and their conservation indicate the potential importance of these molecules in biology.

Indexed as

Open Reading FramesAmino Acid SequenceCysteineHumansModels, MolecularMolecular Sequence DataPeptidesProteomeProteomicsCysteinePeptidesProteome

Identifiers

PMID24152191
PMCPMC3868496

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.