ArticleTissue engineering. Part C, Methods2014
Control of perfusable microvascular network morphology using a multiculture microfluidic system.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
147 citing papers in PubMed.
- Suspended particles for omnidirectional template sacrifice for rapid vascular patterning within engineered tissues.Science advances · 2026Article
- Ex vivo bone marrow subniches influence the fate of human antibody-secreting cells.Science advances · 2026Article
- From flow to form: structuring and patterning hydrogels using microfluidic approaches.Lab on a chip · 2026Review
- Computational fluid dynamics enables predictable scale-up of perfusion bioreactors for microvessel production.PNAS nexus · 2026Article
- Integrating microchannels and flows into 3D printable granular hydrogel matrices.Lab on a chip · 2026Article
- Engineering and Integrating Blood Vessel Organoids: a Comprehensive Blueprint for Physiological and Disease Modeling.Stem cell reviews and reports · 2026Review
- Spontaneous Helical Alignment of Smooth Muscle Cells to Form a Medial Layer for Engineered Microvasculature.Advanced healthcare materials · 2026Article
- Development of a Human 3D Immune-Competent Neurovascular Model Enabling Time-Resolved Monitoring of Neuroinflammatory Dynamics and Neuroimmune Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Roadmap to Perfused Skin: Defining the Next Generation of Research Questions in Cutaneous Tissue Engineering.International journal of molecular sciences · 2026Review
- Spatial distribution and density of fibroblasts determine angiogenic response of endothelial cells.Research square · 2026Article
- Development of a Synthetic Hydrogel to Foster Microvascularization of an Endometriosis Microphysiological System.Advanced healthcare materials · 2026Article
- Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancer.Cancer cell · 2026Article
- Increasing complexity in vascular microphysiological systems: considerations and advances for disease modeling.Frontiers in bioengineering and biotechnology · 2026Review
- Modeling smooth muscle cell-endothelial cell crosstalk in abdominal aortic aneurysms using 3D microvessels on-chip.Vascular biology (Bristol, England) · 2026Article
- Spatial distribution and density of fibroblasts determine angiogenic response of endothelial cells.Microfluidics and nanofluidics · 2026Article
- An Innovative "Tooth-On-Chip" Microfluidic Device Emulating the Structure and Physiology of the Dental Pulp Tissue.Advanced healthcare materials · 2026Article
- Estradiol and dihydrotestosterone exert sex-specific effects on human fibroblast and endothelial proliferation, bioenergetics, and vasculogenesis.Communications biology · 2025Article
- Advanced Brain-on-a-Chip for Wetware Computing: A Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Lung cancer intravasation-on-a-chip: Visualization and machine learning-assisted automatic quantification.Bioactive materials · 2025Article
- Cancer-on-a-chip for precision cancer medicine.Lab on a chip · 2025Review
87 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical and biochemical microenvironment influences the morphological characteristics of microvascular networks (MVNs) formed by endothelial cells (ECs) undergoing the process of vasculogenesis. The objective of this study was to quantify the role of individual factors in determining key network parameters in an effort to construct a set of design principles for engineering vascular networks with prescribed morphologies. To achieve this goal, we developed a multiculture microfluidic platform enabling precise control over paracrine signaling, cell-seeding densities, and hydrogel mechanical properties. Human umbilical vein endothelial cells (HUVECs) were seeded in fibrin gels and cultured alongside human lung fibroblasts (HLFs). The engineered vessels formed in our device contained patent, perfusable lumens. Communication between the two cell types was found to be critical in avoiding network regression and maintaining stable morphology beyond 4 days. The number of branches, average branch length, percent vascularized area, and average vessel diameter were found to depend uniquely on several input parameters. Importantly, multiple inputs were found to control any given output network parameter. For example, the vessel diameter can be decreased either by applying angiogenic growth factors--vascular endothelial growth factor (VEGF) and sphingosine-1-phsophate (S1P)--or by increasing the fibrinogen concentration in the hydrogel. These findings introduce control into the design of MVNs with specified morphological properties for tissue-specific engineering applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.