ArticleProceedings of the National Academy of Sciences of the United States of America2013
Transcript processing and export kinetics are rate-limiting steps in expressing vertebrate segmentation clock genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
- NOTCH1 S2513 is critical for the regulation of NICD levels impacting the segmentation clock in hiPSC-derived PSM cells and somitoids.Genes & development · 2025Article
- Oscillatory control of embryonic development.Development (Cambridge, England) · 2024Review
- Spatiotemporal control of pattern formation during somitogenesis.Science advances · 2024Review
- Amphibian Segmentation Clock Models Suggest How Large Genome and Cell Sizes Slow Developmental Rate.Integrative organismal biology (Oxford, England) · 2024Article
- Stochastic modeling of the mRNA life process: A generalized master equation.Biophysical journal · 2023Article
- Keeping development on time: Insights into post-transcriptional mechanisms driving oscillatory gene expression during vertebrate segmentation.Wiley interdisciplinary reviews. RNA · 2023Review
- Article
- RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer.Nature communications · 2022Article
- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The vertebrate Embryo Clock: Common players dancing to a different beat.Frontiers in cell and developmental biology · 2022Review
- Microfluidics-Based Bioassays and Imaging of Plant Cells.Plant & cell physiology · 2021Review
- Patterning with clocks and genetic cascades: Segmentation and regionalization of vertebrate versus insect body plans.PLoS genetics · 2021Review
- Time and space in segmentation.Interface focus · 2021Article
- Incorporating age and delay into models for biophysical systems.Physical biology · 2021Article
- Pumilio response and AU-rich elements drive rapid decay of Pnrc2-regulated cyclic gene transcripts.Developmental biology · 2020Article
- Dynamics of Notch-Dependent Transcriptional Bursting in Its Native Context.Developmental cell · 2019Article
- CDK1 and CDK2 regulate NICD1 turnover and the periodicity of the segmentation clock.EMBO reports · 2019Article
- Rapid and reversible root growth inhibition by TIR1 auxin signalling.Nature plants · 2018Article
- What does time mean in development?Development (Cambridge, England) · 2018Review
- ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.Development (Cambridge, England) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Sequential production of body segments in vertebrate embryos is regulated by a molecular oscillator (the segmentation clock) that drives cyclic transcription of genes involved in positioning intersegmental boundaries. Mathematical modeling indicates that the period of the clock depends on the total delay kinetics of a negative feedback circuit, including those associated with the synthesis of transcripts encoding clock components [Lewis J (2003) Curr Biol 13(16):1398-1408]. Here, we measure expression delays for three transcripts [Lunatic fringe, Hes7/her1, and Notch-regulated-ankyrin-repeat-protein (Nrarp)], that cycle during segmentation in the zebrafish, chick, and mouse, and provide in vivo measurements of endogenous splicing and export kinetics. We show that mRNA splicing and export are much slower than transcript elongation, with the longest delay (about 16 min in the mouse) being due to mRNA export. We conclude that the kinetics of mRNA and protein production and destruction can account for much of the clock period, and provide strong support for delayed autorepression as the underlying mechanism of the segmentation clock.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.