ArticleThe Plant cell2013
A comprehensive mutational analysis of the Arabidopsis resistance protein RPW8.2 reveals key amino acids for defense activation and protein targeting.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 42 citations in OpenAlex.
- Rice false smut virulence protein subverts host chitin perception and signaling at lemma and palea for floral infection.The Plant cell · 2024Article
- The haustorium: The root of biotrophic fungal pathogens.Frontiers in plant science · 2022Review
- Genome-Wide Association Mapping of Crown and Brown Rust Resistance in Perennial Ryegrass.Genes · 2021Article
- Identification and Characterization ofFrontiers in plant science · 2021Article
- Whole genome resequencing of four Italian sweet pepper landraces provides insights on sequence variation in genes of agronomic value.Scientific reports · 2020Article
- Article
- RPW8/HR repeats control NLR activation in Arabidopsis thaliana.PLoS genetics · 2019Article
- Comparative genome analyses reveal sequence features reflecting distinct modes of host-adaptation between dicot and monocot powdery mildew.BMC genomics · 2018Article
- Arabidopsis phospholipase Dα1 and Dδ oppositely modulate EDS1- and SA-independent basal resistance against adapted powdery mildew.Journal of experimental botany · 2018Article
- Genome reconstruction in Cynara cardunculus taxa gains access to chromosome-scale DNA variation.Scientific reports · 2017Article
- Article
- Homologues of the RPW8 Resistance Protein Are Localized to the Extrahaustorial Membrane that Is Likely Synthesized De Novo.Plant physiology · 2017Article
- Regulated Forms of Cell Death in Fungi.Frontiers in microbiology · 2017Review
- Dual and Opposing Roles of Xanthine Dehydrogenase in Defense-Associated Reactive Oxygen Species Metabolism in Arabidopsis.The Plant cell · 2016Article
- Multiple Evolutionary Events Involved in Maintaining Homologs of Resistance to Powdery Mildew 8 in Brassica napus.Frontiers in plant science · 2016Article
- Biotrophy at Its Best: Novel Findings and Unsolved Mysteries of the Arabidopsis-Powdery Mildew Pathosystem.The arabidopsis book · 2016Article
- The Formation of Stromules In Vitro from Chloroplasts Isolated from Nicotiana benthamiana.PloS one · 2016Article
- Disease Resistance Gene Analogs (RGAs) in Plants.International journal of molecular sciences · 2015Review
- Chloroplast signaling within, between and beyond cells.Frontiers in plant science · 2015Review
- Dominant negative RPW8.2 fusion proteins reveal the importance of haustorium-oriented protein trafficking for resistance against powdery mildew in Arabidopsis.Plant signaling & behavior · 2015Article
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10 authors at 3 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis thaliana resistance to powdery mildew8.2 (RPW8.2) protein is specifically targeted to the extrahaustorial membrane (EHM) encasing the haustorium, or fungal feeding structure, where RPW8.2 activates broad-spectrum resistance against powdery mildew pathogens. How RPW8.2 activates defenses at a precise subcellular locale is not known. Here, we report a comprehensive mutational analysis in which more than 100 RPW8.2 mutants were functionally evaluated for their defense and trafficking properties. We show that three amino acid residues (i.e., threonine-64, valine-68, and aspartic acid-116) are critical for RPW8.2-mediated cell death and resistance to powdery mildew (Golovinomyces cichoracearum UCSC1). Also, we reveal that two arginine (R)- or lysine (K)-enriched short motifs (i.e., R/K-R/K-x-R/K) make up the likely core EHM-targeting signals, which, together with the N-terminal transmembrane domain, define a minimal sequence of 60 amino acids that is necessary and sufficient for EHM localization. In addition, some RPW8.2 mutants localize to the nucleus and/or to a potentially novel membrane that wraps around plastids or plastid-derived stromules. Results from this study not only reveal critical amino acid elements in RPW8.2 that enable haustorium-targeted trafficking and defense, but also provide evidence for the existence of a specific, EHM-oriented membrane trafficking pathway in leaf epidermal cells invaded by powdery mildew.
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