ArticleJournal of biomedical materials research. Part A2014
Crosslinking of collagen scaffolds promotes blood and lymphatic vascular stability.
Article in Journal of biomedical materials research. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Biomaterials in the clinical treatment of lymphedema-a systematic review.Journal of vascular surgery. Venous and lymphatic disorders · 2024Pooled it
- Seeing through collagen: integrative pro-regenerative corneal implants for clearer future.NPJ Regenerative medicine · 2026Review
- Lymphedema: current treatment strategies and future directions in tissue engineering.Frontiers in medicine · 2026Review
- Understanding the Lymphatic System: Tissue-on-Chip Modeling.Annual review of biomedical engineering · 2025Review
- Development of an Easy-to-Fabricate Microdevice for Three-Dimensional Culture and Its Application to Glomerular Endothelial Cell Culture.Micromachines · 2025Article
- Endothelial-mesenchymal transition in skeletal muscle: Opportunities and challenges from 3D microphysiological systems.Bioengineering & translational medicine · 2024Review
- Manufacturing the multiscale vascular hierarchy: progress toward solving the grand challenge of tissue engineering.Trends in biotechnology · 2023Review
- In Vitro Models of Blood and Lymphatic Vessels-Connecting Tissues and Immunity.Advanced biology · 2023Review
- Hydrogels for Exosome Delivery in Biomedical Applications.Gels (Basel, Switzerland) · 2022Review
- Methods for Forming Human Lymphatic Microvessels In Vitro and Assessing their Drainage Function.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Collagen- and hyaluronic acid-based hydrogels and their biomedical applications.Materials science & engineering. R, Reports : a review journal · 2021Article
- Soft Materials by Design: Unconventional Polymer Networks Give Extreme Properties.Chemical reviews · 2021Review
- Kinetics of Postpartum Mesenteric Artery Structure and Function Relative to Pregnancy and Lactation in Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Stromal cell identity modulates vascular morphogenesis in a microvasculature-on-a-chip platform.Lab on a chip · 2021Article
- Microfluidic Biomaterials.Advanced healthcare materials · 2021Review
- Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2021Review
- Engineered Collagen Matrices.Bioengineering (Basel, Switzerland) · 2020Review
- Biomaterial Based Strategies for Engineering New Lymphatic Vasculature.Advanced healthcare materials · 2020Review
- Caught between a "Rho" and a hard place: are CCN1/CYR61 and CCN2/CTGF the arbiters of microvascular stiffness?Journal of cell communication and signaling · 2020Review
- Design and Validation of a Human Brain Endothelial Microvessel-on-a-Chip Open Microfluidic Model Enabling Advanced Optical Imaging.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The low stiffness of reconstituted collagen hydrogels has limited their use as scaffolds for engineering implantable tissues. Although chemical crosslinking has been used to stiffen collagen and protect it against enzymatic degradation in vivo, it remains unclear how crosslinking alters the vascularization of collagen hydrogels. In this study, we examine how the crosslinking agents genipin and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide alter vascular stability and function in microfluidic type I collagen gels in vitro. Under moderate perfusion (∼10 dyn/cm(2) shear stress), tubes of blood endothelial cells (ECs) exhibited indistinguishable stability and barrier function in untreated and crosslinked scaffolds. Surprisingly, under low perfusion (∼5 dyn/cm(2) shear stress) or nearly zero transmural pressure, microvessels in crosslinked scaffolds remained stable, while those in untreated gels rapidly delaminated and became poorly perfused. Similarly, tubes of lymphatic ECs under intermittent flow were more stable in crosslinked gels than in untreated ones. These effects correlated well with the degree of mechanical stiffening, as predicted by analysis of fracture energies at the cell-scaffold interface. This work demonstrates that crosslinking of collagen scaffolds does not hinder normal EC physiology; instead, crosslinked scaffolds promote vascular stability. Thus, routine crosslinking of scaffolds may assist in vascularization of engineered tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.