ArticleHistochemistry and cell biology2014
The molecular basis of the specificity and cross-reactivity of the NeuN epitope of the neuron-specific splicing regulator, Rbfox3.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
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- NeuN expression in health and disease: A histological perspective on neuronal heterogeneity.Histology and histopathology · 2026Review
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- Visualization of the existence of growth hormone secretagogue receptor in the rat nucleus accumbens.Molecular brain · 2024Article
- Neural tissue tolerance to synthetic dural mater graft implantation in a rabbit durotomy model.Journal of toxicologic pathology · 2024Article
- Revisiting the development of cerebellar inhibitory interneurons in the light of single-cell genetic analyses.Histochemistry and cell biology · 2024Review
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- NeuN distribution in brain structures of normal and Zika-infected suckling mice.Journal of molecular histology · 2023Article
- Morphologic Findings in the Cerebral Cortex in COVID-19: Association of Microglial Changes with Clinical and Demographic Variables.Biomedicines · 2023Article
- Ghrelin Regulates Expression of the Transcription Factor Pax6 in Hypoxic Brain Progenitor Cells and Neurons.Cells · 2022Article
- Therapeutic potential of dental pulp stem cell transplantation in a rat model of Alzheimer's disease.Neural regeneration research · 2021Article
- Basic quantitative morphological methods applied to the central nervous system.The Journal of comparative neurology · 2021Review
- Neural-Induced Human Adipose Tissue-Derived Stem Cells Conditioned Medium Ameliorates Rotenone-Induced Toxicity in SH-SY5Y Cells.International journal of molecular sciences · 2021Article
- Characterization of kindled VGAT-Cre mice as a new animal model of temporal lobe epilepsy.Epilepsia · 2020Article
- Differential Glial Activation in Early Epileptogenesis-Insights From Cell-Specific Analysis of DNA Methylation and Gene Expression in the Contralateral Hippocampus.Frontiers in neurology · 2020Article
- The Influence of Murine Genetic Background in Adeno-Associated Virus Transduction of the Mouse Brain.Human gene therapy. Clinical development · 2019Article
- Moving into shape: cell migration during the development and histogenesis of the cerebellum.Histochemistry and cell biology · 2018Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ever since its description and the generation of its defining antibody some 20 years ago, NeuN (Neural Nuclei) has been an invaluable tool for developmental neuroscientist sand neuropathologists to identify neurons and follow their normal or malignant development [corrected].The recent identification of the splicing factor Rbfox3 as the molecule constituting the genuine NeuN epitope has opened up a novel perspective on NeuN immunostaining and its interpretation. Here, we briefly review these recent developments, and we provide a series of data that allow to rationalize the specificity of the NeuN/A60 antibody on aldehyde-fixed tissues on the one hand, and its cross-reactivity with Synapsin I and R3hdm2 on Western blots on the other. We argue that rather than being considered as a mere marker for mature neurons, Rbfox3-mediated NeuN/A60 immunoreactivity may provide a window onto neuronal biology. Specifically, we hypothesize that the phosphorylation-dependent antigenicity of the Rbfox3/NeuN epitope should allow to visualize neuronal physiology realized through Rbfox3, including splicing, on the single-cell level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.