ArticleMatrix biology : journal of the International Society for Matrix Biology2014
Heparin-dependent regulation of fibronectin matrix conformation.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Enhancement of hMSC In Vitro Proliferation by Surface Immobilization of a Heparin-Binding Peptide.Molecules (Basel, Switzerland) · 2023Article
- Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells.Scientific reports · 2022Article
- Heparin and Derivatives for Advanced Cell Therapies.International journal of molecular sciences · 2021Review
- Hypoxia Induced Heparan Sulfate Primes the Extracellular Matrix for Endothelial Cell Recruitment by Facilitating VEGF-Fibronectin Interactions.International journal of molecular sciences · 2019Article
- Fibronectin fiber creep under constant force loading.Acta biomaterialia · 2019Article
- Glycosaminoglycan Neutralization in Coagulation Control.Arteriosclerosis, thrombosis, and vascular biology · 2018Review
- The fibronectin ED-A domain enhances recruitment of latent TGF-β-binding protein-1 to the fibroblast matrix.Journal of cell science · 2018Article
- Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway.Disease markers · 2018Article
- Serum fibronectin distinguishes the early stages of hepatocellular carcinoma.Scientific reports · 2017Article
- Breast cancer cells alter the dynamics of stromal fibronectin-collagen interactions.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Fibronectin fibrils regulate TGF-β1-induced Epithelial-Mesenchymal Transition.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- The provisional matrix: setting the stage for tissue repair outcomes.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- Fibronectin promotes directional persistence in fibroblast migration through interactions with both its cell-binding and heparin-binding domains.Scientific reports · 2017Article
- Plasma fibronectin stabilizesProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Sulfated hyaluronan alters fibronectin matrix assembly and promotes osteogenic differentiation of human bone marrow stromal cells.Scientific reports · 2016Article
- Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.The Journal of biological chemistry · 2016Article
- Extracellular matrix stiffness modulates VEGF calcium signaling in endothelial cells: individual cell and population analysis.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix.Nature communications · 2015Article
- Fibronectin Mechanobiology Regulates Tumorigenesis.Cellular and molecular bioengineeringReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Extracellular matrix (ECM) conformation is regulated by a variety of stimuli in vivo, including mechanical forces and allosteric binding partners, and these conformational changes contribute to the regulation of cell behavior. Heparin and heparan sulfate, for example, have been shown to regulate the sequestration and presentation of numerous growth factors, including vascular endothelial growth factor, on the heparin 2 binding domain in fibronectin (Fn). However, mechanical force also alters Fn conformation, indicating that the growth factor binding region may be co-regulated by both heparin and mechanical force. Herein, we describe a simple antibody-based method for evaluating the conformation of the heparin 2 binding domain in Fn, and use it to determine the relative contributions of heparin and mechanical strain to the regulation of Fn conformation. We achieved specificity in quantifying conformational changes in this region of Fn by measuring the ratio of two fluorescent monoclonal antibodies, one that is insensitive to Fn conformational changes and a second whose binding is reduced or enhanced by non-equilibrium conformational changes. Importantly, this technique is shown to work on Fn adsorbed on surfaces, single Fn fibers, and Fn matrix fibers in cell culture. Using our dual antibody approach, we show that heparin and mechanical strain co-regulate Fn conformation in matrix fibrils, which is the first demonstration of heparin-dependent regulation of Fn in its physiologically-relevant fibrillar state. Furthermore, the dual antibody approach utilizes commercially available antibodies and simple immunohistochemistry, thus making it accessible to a wide range of scientists interested in Fn mechanobiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.