ArticlePLoS neglected tropical diseases2013
Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Identifying Leptospira interrogans putative virulence factors with a yeast protein expression screen.Applied microbiology and biotechnology · 2022Article
- Role of Diagnostics in Epidemiology, Management, Surveillance, and Control of Leptospirosis.Pathogens (Basel, Switzerland) · 2022Review
- Enzyme immunoassays (EIA) for serodiagnosis of human leptospirosis: specific IgG3/IgG1 isotyping may further inform diagnosis of acute disease.PLoS neglected tropical diseases · 2022Article
- Evaluation of a genus-specific rGroELScientific reports · 2021Article
- Article
- APRANK: Computational Prioritization of Antigenic Proteins and Peptides From Complete Pathogen Proteomes.Frontiers in immunology · 2021Article
- Leptospiral Immunoglobulin-Like Domain Proteins: Roles in Virulence and Immunity.Frontiers in immunology · 2020Review
- An Improved Enzyme-Linked Immunoassay for the Detection ofThe American journal of tropical medicine and hygiene · 2018Article
- Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression.Scientific reports · 2018Article
- Children with cerebral malaria or severe malarial anaemia lack immunity to distinct variant surface antigen subsets.Scientific reports · 2018Article
- Prospective evaluation of accuracy and clinical utility of the Dual Path Platform (DPP) assay for the point-of-care diagnosis of leptospirosis in hospitalized patients.PLoS neglected tropical diseases · 2018Article
- Identification ofClinical proteomics · 2018Article
- Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis.PLoS neglected tropical diseases · 2017Article
- A Novel Pan-Genome Reverse Vaccinology Approach Employing a Negative-Selection Strategy for Screening Surface-Exposed Antigens againstFrontiers in microbiology · 2017Article
- Discovery of Novel Leptospirosis Vaccine Candidates Using Reverse and Structural Vaccinology.Frontiers in immunology · 2017Article
- A targeted immunomic approach identifies diagnostic antigens in the human pathogen Babesia microti.Transfusion · 2016Article
- Rapid and Sensitive Multiplex Detection of Burkholderia pseudomallei-Specific Antibodies in Melioidosis Patients Based on a Protein Microarray Approach.PLoS neglected tropical diseases · 2016Article
- Protein microarray-mediated detection of antienterovirus antibodies in serum.The Journal of international medical research · 2016Article
- The Miller Hypothesis.Forum on immunopathological diseases and therapeutics · 2016Article
- In Vivo-Expressed Proteins of Virulent Leptospira interrogans Serovar Autumnalis N2 Elicit Strong IgM Responses of Value in Conclusive Diagnosis.Clinical and vaccine immunology : CVI · 2016Article
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundLeptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease. METHODOLOGY: In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line blot assay was performed to validate the accuracy of the protein microarray results. PRINCIPAL
findingsWe found 16 antigens that can discriminate between acute cases and healthy individuals from a region with high endemic transmission of leptospirosis, and 18 antigens that distinguish convalescent cases. Some of the antigens identified in this study, such as LipL32, the non-identical domains of the Lig proteins, GroEL, and Loa22 are already known to be recognized by sera from human patients, thus serving as proof-of-concept for the serodiagnostic antigen discovery approach. Several novel antigens were identified, including the hypothetical protein LIC10215 which showed good sensitivity and specificity rates for both acute- and convalescent-phase patients.
conclusionsOur study is the first large-scale evaluation of immunodominant antigens associated with naturally acquired leptospiral infection, and novel as well as known serodiagnostic leptospiral antigens that are recognized by antibodies in the sera of leptospirosis cases were identified. The novel antigens identified here may have potential use in both the development of new tests and the improvement of currently available assays for diagnosing this neglected tropical disease. Further research is needed to assess the utility of these antigens in more deployable diagnostic platforms.
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