ArticlePloS one2013
Interkingdom complementation reveals structural conservation and functional divergence of 14-3-3 proteins.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Redundant and Distinct Roles of Two 14-3-3 Proteins inJournal of fungi (Basel, Switzerland) · 2024Article
- The Lifespan ofInsects · 2024Article
- Domesticated LTR-RetrotransposonLife (Basel, Switzerland) · 2022Review
- 14-3-3ζ-A Novel Immunogen Promotes Inflammatory Cytokine Production.Frontiers in immunology · 2019Article
- The 14-3-3 Protein Homolog ArtA Regulates Development and Secondary Metabolism in the Opportunistic Plant Pathogen Aspergillus flavus.Applied and environmental microbiology · 2018Article
- GFAP expression in injured astrocytes in rats.Experimental and therapeutic medicine · 2017Article
- Bioinformatic analysis reveals new determinants of antigenic 14-3-3 proteins and a novel antifungal strategy.PloS one · 2017Article
- 14-3-3σ is an independent prognostic biomarker for gastric cancer and is associated with apoptosis and proliferation in gastric cancer.Oncology letters · 2015Article
- The crystal structure of Giardia duodenalis 14-3-3 in the apo form: when protein post-translational modifications make the difference.PloS one · 2014Article
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5 authors.
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Abstract
The 14-3-3s are small acidic cytosolic proteins that interact with multiple clients and participate in essential cellular functions in all eukaryotes. Available structural and functional information about 14-3-3s is largely derived from higher eukaryotes, which contain multiple members of this protein family suggesting functional specialization. The exceptional sequence conservation among 14-3-3 family members from diverse species suggests a common ancestor for 14-3-3s, proposed to have been similar to modern 14-3-3ε isoforms. Structural features of the sole family member from the protozoan Giardia duodenalis (g14-3-3), are consistent with this hypothesis, but whether g14-3-3 is functionally homologous to the epsilon isoforms is unknown. We use inter-kingdom reciprocal functional complementation and biochemical methods to determine whether g14-3-3 is structurally and functionally homologous with members of the two 14-3-3 conservation groups of the metazoan Drosophila melanogaster. Our results indicate that although g14-3-3 is structurally homologous to D14-3-3ε, functionally it diverges presenting characteristics of other 14-3-3s. Given the basal position of Giardia in eukaryotic evolution, this finding is consistent with the hypothesis that 14-3-3ε isoforms are ancestral to other family members.
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