ArticlePloS one2013
Identification and characterization of a novel trehalose synthase gene derived from saline-alkali soil metagenomes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 48 citations in OpenAlex.
- Structural and Mutational Analyses of Trehalose Synthase fromJournal of agricultural and food chemistry · 2024Article
- Characterization of cold-active trehalose synthase from Pseudarthrobacter sp. for trehalose bioproduction.Bioresources and bioprocessing · 2023Article
- Integrated Whole-Cell Biocatalysis for Trehalose Production from Maltose Using PermeabilizedJournal of microbiology and biotechnology · 2022Article
- A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose.Microbiology spectrum · 2021Article
- Glycogen metabolism of the anammox bacterium "Candidatus Brocadia sinica".The ISME journal · 2021Article
- The diversity and commonalities of the radiation-resistance mechanisms of Deinococcus and its up-to-date applications.AMB Express · 2019Review
- The Production of ACC Deaminase and Trehalose by the Plant Growth Promoting BacteriumFrontiers in microbiology · 2019Article
- Improvement of Trehalose Production by Immobilized Trehalose Synthase fromMolecules (Basel, Switzerland) · 2018Article
- Improvement of Lead Tolerance of Saccharomyces cerevisiae by Random Mutagenesis of Transcription Regulator SPT3.Applied biochemistry and biotechnology · 2018Article
- Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134.Scientific reports · 2017Article
- Captured metagenomics: large-scale targeting of genes based on 'sequence capture' reveals functional diversity in soils.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli.PloS one · 2015Article
- Structures of trehalose synthase from Deinococcus radiodurans reveal that a closed conformation is involved in catalysis of the intramolecular isomerization.Acta crystallographica. Section D, Biological crystallography · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel trehalose synthase (TreS) gene was identified from a metagenomic library of saline-alkali soil by a simple activity-based screening system. Sequence analysis revealed that TreS encodes a protein of 552 amino acids, with a deduced molecular weight of 63.3 kDa. After being overexpressed in Escherichia coli and purified, the enzymatic properties of TreS were investigated. The recombinant TreS displayed its optimal activity at pH 9.0 and 45 °C, and the addition of most common metal ions (1 or 30 mM) had no inhibition effect on the enzymatic activity evidently, except for the divalent metal ions Zn(2+) and Hg(2+). Kinetic analysis showed that the recombinant TreS had a 4.1-fold higher catalytic efficiency (Kcat/K m ) for maltose than for trehalose. The maximum conversion rate of maltose into trehalose by the TreS was reached more than 78% at a relatively high maltose concentration (30%), making it a good candidate in the large-scale production of trehalsoe after further study. In addition, five amino acid residues, His172, Asp201, Glu251, His318 and Asp319, were shown to be conserved in the TreS, which were also important for glycosyl hydrolase family 13 enzyme catalysis.
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