ArticlePloS one2013
Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 64 citations in OpenAlex.
- Comparative Expression of Diacylglycerol Acyltransferases for Enhanced Accumulation of Punicic Acid-Enriched Triacylglycerols inMolecules (Basel, Switzerland) · 2026Article
- Expression of the peanut diacylglycerol acyltransferase 3 increases the neutral lipid content and improves the fatty acid composition ofFrontiers in plant science · 2026Article
- Functional diversity of sunflower DGAT1 and DGAT2 enzymes underlying seed triacylglycerol biosynthesis.Frontiers in plant science · 2026Article
- The Diacylglycerol Acyltransferase 3 ofBiomolecules · 2025Article
- Functional Characterization of the Effects of CsDGAT1 and CsDGAT2 on Fatty Acid Composition inInternational journal of molecular sciences · 2024Article
- Ectopic Expression ofPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Identification, Classification, and Expression Analyses of theInternational journal of molecular sciences · 2023Article
- Editorial: Woody oil crops: key trait formation and regulation.Frontiers in plant science · 2023Article
- Tung tree stearoyl-acyl carrier protein Δ9 desaturase improves oil content and cold resistance ofFrontiers in plant science · 2023Article
- Increase in alpha-linolenic acid content by simultaneous expression of fatty acid metabolism genes in Sesame (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Cottonseed extracts regulate gene expression in human colon cancer cells.Scientific reports · 2022Article
- Functional analysis of alternative castor bean DGAT enzymes.Genetics and molecular biology · 2022Article
- Acyl-CoA-dependent and acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols.Frontiers in plant science · 2022Article
- Ectopic overexpression of a type-II DGAT (CeDGAT2-2) derived from oil-rich tuber of Cyperus esculentus enhances accumulation of oil and oleic acid in tobacco leaves.Biotechnology for biofuels · 2021Article
- Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells.Scientific reports · 2021Article
- Tung Tree (Vernicia fordii) Genome Provides A Resource for Understanding Genome Evolution and Improved Oil Production.Genomics, proteomics & bioinformatics · 2019Article
- Genome-Wide Identification and Characterization ofInternational journal of molecular sciences · 2019Article
- Identification of the major diacylglycerol acyltransferase mRNA in mouse adipocytes and macrophages.BMC biochemistry · 2018Article
- Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis.Scientific reports · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diacylglycerol acyltransferases (DGAT) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. DGAT genes have been identified in numerous organisms. Multiple isoforms of DGAT are present in eukaryotes. We previously cloned DGAT1 and DGAT2 genes of tung tree (Vernicia fordii), whose novel seed TAGs are useful in a wide range of industrial applications. The objective of this study was to understand the developmental regulation of DGAT family gene expression in tung tree. To this end, we first cloned a tung tree gene encoding DGAT3, a putatively soluble form of DGAT that possesses 11 completely conserved amino acid residues shared among 27 DGAT3s from 19 plant species. Unlike DGAT1 and DGAT2 subfamilies, DGAT3 is absent from animals. We then used TaqMan and SYBR Green quantitative real-time PCR, along with northern and western blotting, to study the expression patterns of the three DGAT genes in tung tree tissues. Expression results demonstrate that 1) all three isoforms of DGAT genes are expressed in developing seeds, leaves and flowers; 2) DGAT2 is the major DGAT mRNA in tung seeds, whose expression profile is well-coordinated with the oil profile in developing tung seeds; and 3) DGAT3 is the major form of DGAT mRNA in tung leaves, flowers and immature seeds prior to active tung oil biosynthesis. These results suggest that DGAT2 is probably the major TAG biosynthetic isoform in tung seeds and that DGAT3 gene likely plays a significant role in TAG metabolism in other tissues. Therefore, DGAT2 should be a primary target for tung oil engineering in transgenic organisms.
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