ArticlePloS one2013
2, 6-Dichlorobenzonitrile causes multiple effects on pollen tube growth beyond altering cellulose synthesis in Pinus bungeana Zucc.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Low-temperature stress modulates pollen tube growth through temperature-dependent multi-level regulatory mechanisms in Camellia sinensis.Plant reproduction · 2026Article
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Nitric Oxide Participates in Aluminum-Stress-Induced Pollen Tube Growth Inhibition in Tea (Plants (Basel, Switzerland) · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Effect of boron toxicity on pollen tube cell wall architecture and the relationship of cell wall components ofFrontiers in plant science · 2022Article
- Effect of methyl jasmonate on in-vitro pollen germination and tube elongation of Pinus nigra.Protoplasma · 2020Article
- Putrescine modifies the pollen tube growth of tea (Camellia sinensis) by affecting actin organization and cell wall structure.Protoplasma · 2020Article
- Evolution of Cell Wall Polymers in Tip-Growing Land Plant Gametophytes: Composition, Distribution, Functional Aspects and Their Remodeling.Frontiers in plant science · 2019Review
- Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens.Scientific reports · 2018Article
- Article
- Boron Toxicity Causes Multiple Effects on Malus domestica Pollen Tube Growth.Frontiers in plant science · 2016Article
- Cellular and Subcellular Immunohistochemical Localization and Quantification of Cadmium Ions in Wheat (Triticum aestivum).PloS one · 2015Article
- No stress! Relax! Mechanisms governing growth and shape in plant cells.International journal of molecular sciences · 2014Review
- Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulose is an important component of cell wall, yet its location and function in pollen tubes remain speculative. In this paper, we studied the role of cellulose synthesis in pollen tube elongation in Pinus bungeana Zucc. by using the specific inhibitor, 2, 6-dichlorobenzonitrile (DCB). In the presence of DCB, the growth rate and morphology of pollen tubes were distinctly changed. The organization of cytoskeleton and vesicle trafficking were also disturbed. Ultrastructure of pollen tubes treated with DCB was characterized by the loose tube wall and damaged organelles. DCB treatment induced distinct changes in tube wall components. Fluorescence labeling results showed that callose, and acidic pectin accumulated in the tip regions, whereas there was less cellulose when treated with DCB. These results were confirmed by FTIR microspectroscopic analysis. In summary, our findings showed that inhibition of cellulose synthesis by DCB affected the organization of cytoskeleton and vesicle trafficking in pollen tubes, and induced changes in the tube wall chemical composition in a dose-dependent manner. These results confirm that cellulose is involved in the establishment of growth direction of pollen tubes, and plays important role in the cell wall construction during pollen tube development despite its lower quantity.
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