ArticlePloS one2013
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by Escherichia coli in human and environmental samples.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Antibody-based alternatives to animal testing for toxin detection and antitoxin evaluation.Frontiers in toxicology · 2025Review
- A novel Shiga toxin 2a neutralizing antibody therapeutic with low immunogenicity and high efficacy.Antimicrobial agents and chemotherapy · 2024Article
- Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-Producing Escherichia coli Outer Membrane Vesicles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Validation of a Cell-Based Assay for Detection of Active Shiga Toxins Produced byInternational journal of environmental research and public health · 2020Article
- Structural and Functional Characterization of Stx2k, a New Subtype of Shiga Toxin 2.Microorganisms · 2019Article
- Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats.Scientific reports · 2018Article
- Article
- Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury.PLoS pathogens · 2017Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Development of a Sandwich ELISA for EHEC O157:H7 Intimin γ1.PloS one · 2016Article
- A New Immunoassay for Detecting All Subtypes of Shiga Toxins Produced by Shiga Toxin-Producing E. coli in Ground Beef.PloS one · 2016Article
- Mass Spectrometry-Based Method of Detecting and Distinguishing Type 1 and Type 2 Shiga-Like Toxins in Human Serum.Toxins · 2015Article
- An in vitro combined antibiotic-antibody treatment eliminates toxicity from Shiga toxin-producing Escherichia coli.Antimicrobial agents and chemotherapy · 2015Article
- Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain.Scientific reports · 2015Article
- Salmonella, Shigella, and yersinia.Clinics in laboratory medicine · 2015Review
- Shiga toxin 2 subtypes of enterohemorrhagic E. coli O157:H- E32511 analyzed by RT-qPCR and top-down proteomics using MALDI-TOF-TOF-MS.Journal of the American Society for Mass Spectrometry · 2015Article
- New Stx2e Monoclonal Antibodies for Immunological Detection and Distinction of Stx2 Subtypes.PloS one · 2015Article
- Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.The Analyst · 2014Article
- A high-throughput, precipitating colorimetric sandwich ELISA microarray for Shiga toxins.Toxins · 2014Article
- New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundShiga toxin-producing Escherichia coli (STEC) are frequent causes of severe human diseases ranging from diarrhea to hemolytic uremic syndrome. The existing strategy for detection of STEC relies on the unique sorbitol-negative fermentation property of the O157 strains, the most commonly identified serotype has been E. coli O157. It is becoming increasingly evident, however, that numerous non-O157 STEC serotypes also cause outbreaks and severe illnesses. It is necessary to have new methods that are capable of detecting all STEC strains. METHODS AND
findingsHere we describe the development of a sandwich ELISA assay for detecting both O157 and non-O157 STECs by incorporating a novel polyclonal antibody (pAb) against Stx2. The newly established immunoassay was capable of detecting Stx2a spiked in environmental samples with a limit of detection between 10 and 100 pg/mL in soil and between 100 and 500 pg/mL in feces. When applied to 36 bacterial strains isolated from human and environmental samples, this assay detected Stx2 in all strains that were confirmed to be stx2-positive by real-time PCR, demonstrating a 100% sensitivity and specificity.
conclusionsThe sandwich ELISA developed in this study will enable any competent laboratory to identify and characterize Stx2-producing O157 and non-O157 strains in human and environmental samples, resulting in rapid diagnosis and patient care. The results of epitope mapping from this study will be useful for further development of a peptide-based antibody and vaccine.
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