ArticlePloS one2013
Super-resolution imaging of bacteria in a microfluidics device.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- RNA polymerase redistribution supports growth in E. coli strains with a minimal number of rRNA operons.Nucleic acids research · 2023Article
- Can super-resolution microscopy become a standard characterization technique for materials chemistry?Chemical science · 2022Review
- Practical observations on the use of fluorescent reporter systems in Clostridioides difficile.Antonie van Leeuwenhoek · 2022Article
- Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel.Scientific reports · 2021Article
- Comparison of Escherichia coli surface attachment methods for single-cell microscopy.Scientific reports · 2019Article
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- Integrated multi-channel optical system for bacteria characterization and its potential use for monitoring of environmental bacteria.Biomedical optics express · 2019Article
- Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating.PloS one · 2019Article
- A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels.Scientific reports · 2018Article
- Microfluidic Based Optical Microscopes on Chip.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Review
- TADs are 3D structural units of higher-order chromosome organization inScience advances · 2018Article
- Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective.Frontiers in microbiology · 2018Review
- Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions.Nature communications · 2017Article
- Angular reconstitution-based 3D reconstructions of nanomolecular structures from superresolution light-microscopy images.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Direct observation of the translocation mechanism of transcription termination factor Rho.Nucleic acids research · 2015Article
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Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria have evolved complex, highly-coordinated, multi-component cellular engines to achieve high degrees of efficiency, accuracy, adaptability, and redundancy. Super-resolution fluorescence microscopy methods are ideally suited to investigate the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. These techniques require the long-term stability of samples, high signal-to-noise-ratios, low chromatic aberrations and surface flatness, conditions difficult to meet with traditional immobilization methods. We present a method in which cells are functionalized to a microfluidics device and fluorophores are injected and imaged sequentially. This method has several advantages, as it permits the long-term immobilization of cells and proper correction of drift, avoids chromatic aberrations caused by the use of different filter sets, and allows for the flat immobilization of cells on the surface. In addition, we show that different surface chemistries can be used to image bacteria at different time-scales, and we introduce an automated cell detection and image analysis procedure that can be used to obtain cell-to-cell, single-molecule localization and dynamic heterogeneity as well as average properties at the super-resolution level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.