ArticlePloS one2013
Targeted gene deletion of miRNAs in mice by TALEN system.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- An efficient evaluation system for factors affecting the genome editing efficiency in mouse.Experimental animals · 2023Article
- Review
- Methods to Investigate miRNA Function: Focus on Platelet Reactivity.Thrombosis and haemostasis · 2021Review
- Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production.Plant biotechnology journal · 2020Article
- Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human.Frontiers in physiology · 2019Article
- Ablation of miR-146b in mice causes hematopoietic malignancy.Blood advances · 2018Article
- Peptidyl arginine deiminase 2 (Padi2) is expressed in Sertoli cells in a specific manner and regulated by SOX9 during testicular development.Scientific reports · 2018Article
- Selected microRNA-192 mutant indicates association with several function genes in bovine cells.Genes & genomics · 2018Article
- Insight into the molecular mechanism of miR-192 regulatingBioscience reports · 2018Article
- The in vivo genetic toolkit for studying expression and functions of Drosophila melanogaster microRNAs.RNA biology · 2017Review
- Current Status and Strategy of microRNA Research for Cartilage Development and Osteoarthritis Pathogenesis.Journal of bone metabolism · 2016Review
- CRISPR/cas9, a novel genomic tool to knock down microRNA in vitro and in vivo.Scientific reports · 2016Article
- Review
- The Development of TALE Nucleases for Biotechnology.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9.Scientific reports · 2015Article
- TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis.Stem cells and development · 2015Article
- Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems.Scientific reports · 2014Article
- Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system.Scientific reports · 2014Article
- TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys.Cell stem cell · 2014Article
- An image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level.F1000Research · 2014Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Mice are among the most valuable model animal species with an enormous amount of heritage in genetic modification studies. However, targeting genes in mice is sometimes difficult, especially for small genes, such as microRNAs (miRNAs) and targeting genes in repeat sequences. Here we optimized the application of TALEN system for mice and successfully obtained gene targeting technique in mice for intergenic region and series of microRNAs. Microinjection of synthesized RNA of TALEN targeting each gene in one cell stage of embryo was carried out and injected oocytes were transferred into pseudopregnant ICR female mice, producing a high success rate of the targeted deletion of miRNA genes. In our condition, TALEN RNA without poly(A) tail worked better than that of with poly(A) tail. This mutated allele in miRNA was transmitted to the next generation, suggesting the successful germ line transmission of this targeting method. Consistent with our notion of miRNAs maturation mechanism, in homozygous mutant mice of miR-10a, the non- mutated strand of miRNAs expression was completely diminished. This method will lead us to expand and accelerate our genetic research using mice in a high throughput way.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.