ArticlePloS one2013
DNA analysis of herbarium Specimens of the grass weed Alopecurus myosuroides reveals herbicide resistance pre-dated herbicides.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- The genetic architecture and spatiotemporal dynamics of adaptation across human-modified landscapes.The New phytologist · 2026Review
- Population genomics reveal multiple independent origins of pesticide resistance in the polyphagous pest, Tetranychus urticae.Communications biology · 2025Article
- Theoretical assessment of persistence and adaptation in weeds with complex life cycles.Nature plants · 2023Article
- Deep haplotype analyses of target-site resistance locus ACCase in blackgrass enabled by pool-based amplicon sequencing.Plant biotechnology journal · 2023Article
- Standing genetic variation fuels rapid evolution of herbicide resistance in blackgrass.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genomic-based epidemiology reveals independent origins and gene flow of glyphosate resistance in Bassia scoparia populations across North America.Molecular ecology · 2021Article
- The Global Museum: natural history collections and the future of evolutionary science and public education.PeerJ · 2020Article
- Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment.PLoS pathogens · 2019Article
- The evolutionary origins of pesticide resistance.Biological reviews of the Cambridge Philosophical Society · 2019Article
- The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora.Frontiers in plant science · 2018Review
- Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing.Frontiers in plant science · 2018Article
- Genetic diversity and structure ofEvolutionary applications · 2017Article
- Effect of herbicide resistance endowing Ile-1781-Leu and Asp-2078-Gly ACCase gene mutations on ACCase kinetics and growth traits in Lolium rigidum.Journal of experimental botany · 2015Article
- The use of museum specimens with high-throughput DNA sequencers.Journal of human evolution · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetyl-CoA carboxylase (ACCase) alleles carrying one point mutation that confers resistance to herbicides have been identified in arable grass weed populations where resistance has evolved under the selective pressure of herbicides. In an effort to determine whether herbicide resistance evolves from newly arisen mutations or from standing genetic variation in weed populations, we used herbarium specimens of the grass weed Alopecurus myosuroides to seek mutant ACCase alleles carrying an isoleucine-to-leucine substitution at codon 1781 that endows herbicide resistance. These specimens had been collected between 1788 and 1975, i.e., prior to the commercial release of herbicides inhibiting ACCase. Among the 734 specimens investigated, 685 yielded DNA suitable for PCR. Genotyping the ACCase locus using the derived Cleaved Amplified Polymorphic Sequence (dCAPS) technique identified one heterozygous mutant specimen that had been collected in 1888. Occurrence of a mutant codon encoding a leucine residue at codon 1781 at the heterozygous state was confirmed in this specimen by sequencing, clearly demonstrating that resistance to herbicides can pre-date herbicides in weeds. We conclude that point mutations endowing resistance to herbicides without having associated deleterious pleiotropic effects can be present in weed populations as part of their standing genetic variation, in frequencies higher than the mutation frequency, thereby facilitating their subsequent selection by herbicide applications.
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