ArticlePLoS genetics2013
The transacting factor CBF-A/Hnrnpab binds to the A2RE/RTS element of protamine 2 mRNA and contributes to its translational regulation during mouse spermatogenesis.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Research Progress on the Structural and Functional Roles of hnRNPs in Muscle Development.Biomolecules · 2023Review
- Decoding the Spermatogenesis Program: New Insights from Transcriptomic Analyses.Annual review of genetics · 2022Review
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- hnRNP A/B Proteins: An Encyclopedic Assessment of Their Roles in Homeostasis and Disease.Biology · 2021Review
- Article
- Cellular hnRNPAB binding to viral nucleoprotein inhibits flu virus replication by blocking nuclear export of viral mRNA.iScience · 2021Article
- Functional characterization of testis-brain RNA-binding protein, TB-RBP/Translin, in translational regulation.The Journal of reproduction and development · 2021Article
- Ciphers and Executioners: How 3'-Untranslated Regions Determine the Fate of Messenger RNAs.Frontiers in genetics · 2019Review
- Mouse TMCO5 is localized to the manchette microtubules involved in vesicle transfer in the elongating spermatids.PloS one · 2019Article
- A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.Developmental cell · 2018Article
- Infection of epididymal epithelial cells and leukocytes drives seminal shedding of Zika virus in a mouse model.PLoS neglected tropical diseases · 2018Article
- A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model.Journal of visualized experiments : JoVE · 2018Article
- Sorting mRNA Molecules for Cytoplasmic Transport and Localization.Frontiers in genetics · 2018Review
- Unraveling transcriptome dynamics in human spermatogenesis.Development (Cambridge, England) · 2017Article
- Gene networks for total number born in pigs across divergent environments.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Article
- Imaging mRNA and protein interactions within neurons.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Drosophila LKB1 is required for the assembly of the polarized actin structure that allows spermatid individualization.PloS one · 2017Article
- CTCF contributes in a critical way to spermatogenesis and male fertility.Scientific reports · 2016Article
- Differential Expression of Long Noncoding RNAs between Sperm Samples from Diabetic and Non-Diabetic Mice.PloS one · 2016Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During spermatogenesis, mRNA localization and translation are believed to be regulated in a stage-specific manner. We report here that the Protamine2 (Prm2) mRNA transits through chromatoid bodies of round spermatids and localizes to cytosol of elongating spermatids for translation. The transacting factor CBF-A, also termed Hnrnpab, contributes to temporal regulation of Prm2 translation. We found that CBF-A co-localizes with the Prm2 mRNA during spermatogenesis, directly binding to the A2RE/RTS element in the 3' UTR. Although both p37 and p42 CBF-A isoforms interacted with RTS, they associated with translationally repressed and de-repressed Prm2 mRNA, respectively. Only p42 was found to interact with the 5'cap complex, and to co-sediment with the Prm2 mRNA in polysomes. In CBF-A knockout mice, expression of protamine 2 (PRM2) was reduced and the Prm2 mRNA was prematurely translated in a subset of elongating spermatids. Moreover, a high percentage of sperm from the CBF-A knockout mouse showed abnormal DNA morphology. We suggest that CBF-A plays an important role in spermatogenesis by regulating stage-specific translation of testicular mRNAs.
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