ArticleMolecular biotechnology2014
Assessment of reference genes for real-time quantitative PCR gene expression normalization during C2C12 and H9c2 skeletal muscle differentiation.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
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17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
- Expression of genes in the skeletal muscle of individuals with cachexia/sarcopenia: A systematic review.PloS one · 2019Pooled it
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- In vitro endocrine and cardiometabolic toxicity associated with artificial turf materials.Environmental toxicology and pharmacology · 2024Article
- Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNA.Nature structural & molecular biology · 2024Article
- Oral Administration of Rhamnan Sulfate fromCells · 2023Article
- Identification of Maleimide-Fused Carbazoles as Novel Noncanonical Bone Morphogenetic Protein Synergizers.ACS pharmacology & translational science · 2023Article
- Screening and validation of reference genes for qRT-PCR of bovine skeletal muscle-derived satellite cells.Scientific reports · 2022Article
- Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms.PeerJ · 2022Article
- Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica.Molecular biology reports · 2021Article
- AlteredDisease models & mechanisms · 2020Article
- Article
- Validation of Internal Control Genes for Quantitative Real-Time PCR Gene Expression Analysis inMolecules (Basel, Switzerland) · 2018Article
- The role of acid-base imbalance in statin-induced myotoxicity.Translational research : the journal of laboratory and clinical medicine · 2016Article
- Selection of reference genes for microRNA quantitative expression analysis in Chinese perch, Siniperca chuatsi.International journal of molecular sciences · 2015Article
- Identification and validation of quantitative PCR reference genes suitable for normalizing expression in normal and dystrophic cell culture models of myogenesis.PLoS currents · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Skeletal muscle differentiation occurs during muscle development and regeneration. To initiate and maintain the differentiated state, a multitude of gene expression changes occur. Accurate assessment of these differentiation-related gene expression changes requires good quality template, but more specifically, appropriate internal controls for normalization. Two cell line-based models used for in vitro analyses of muscle differentiation incorporate mouse C2C12 and rat H9c2 cells. In this study, we set out to identify the most appropriate controls for mRNA expression normalization during C2C12 and H9c2 differentiation. We assessed the expression profiles of Actb, Gapdh, Hprt, Rps12 and Tbp during C2C12 differentiation and of Gapdh and Rps12 during H9c2 differentiation. Using NormFinder, we validated the stability of the genes individually and of the geometric mean generated from different gene combinations. We verified our results using Myogenin. Our study demonstrates that using the geometric mean of a combination of specific reference genes for normalization provides a platform for more precise test gene expression assessment during myoblast differentiation than using the absolute expression value of an individual gene and reinforces the necessity of reference gene validation.
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