ArticleJournal of visualized experiments : JoVE2013
A decellularization methodology for the production of a natural acellular intestinal matrix.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Direct Extracellular Matrix Modulation Attenuates Intestinal Fibrosis via a Fibronectin-Targeted Approach.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The functional role of the extracellular matrix in inflammatory bowel disease associated gut fibrosis.Matrix biology : journal of the International Society for Matrix Biology · 2025Review
- Article
- Decellularization of the human urethra for tissue engineering applications.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- Nerve regeneration using decellularized tissues: challenges and opportunities.Frontiers in neuroscience · 2023Review
- Small-diameter artery decellularization: Effects of anionic detergent concentration and treatment duration on porcine internal thoracic arteries.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Article
- Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds.International journal of molecular sciences · 2020Review
- A Comparison of Sterilization Techniques for Production of Decellularized Intestine in Mice.Tissue engineering. Part C, Methods · 2020Article
- Decellularization techniques and their applications for the repair and regeneration of the nervous system.Methods (San Diego, Calif.) · 2020Review
- Human Microphysiological Models of Intestinal Tissue and Gut Microbiome.Frontiers in bioengineering and biotechnology · 2020Review
- Evaluation of a Miniaturized Biologically Vascularized Scaffold in vitro and in vivo.Scientific reports · 2018Article
- Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome.BioMed research international · 2018Article
- Tissue engineering for the treatment of short bowel syndrome in children.Pediatric research · 2018Review
- Advancing Intestinal Organoid Technology Toward Regenerative Medicine.Cellular and molecular gastroenterology and hepatology · 2018Review
- Extracellular Matrix Bioscaffolds for Building Gastrointestinal Tissue.Cellular and molecular gastroenterology and hepatology · 2018Review
- The extracellular matrix of the gastrointestinal tract: a regenerative medicine platform.Nature reviews. Gastroenterology & hepatology · 2017Review
- Stem cell-mediated functionalization of titanium implants.Journal of materials science. Materials in medicine · 2017Article
- Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications.BioMed research international · 2017Review
- Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application.PloS one · 2017Article
- Generation of an artificial intestine for the management of short bowel syndrome.Current opinion in organ transplantation · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Successful tissue engineering involves the combination of scaffolds with appropriate cells in vitro or in vivo. Scaffolds may be synthetic, naturally-derived or derived from tissues/organs. The latter are obtained using a technique called decellularization. Decellularization may involve a combination of physical, chemical, and enzymatic methods. The goal of this technique is to remove all cellular traces whilst maintaining the macro- and micro-architecture of the original tissue. Intestinal tissue engineering has thus far used relatively simple scaffolds that do not replicate the complex architecture of the native organ. The focus of this paper is to describe an efficient decellularization technique for rat small intestine. The isolation of the small intestine so as to ensure the maintenance of a vascular connection is described. The combination of chemical and enzymatic solutions to remove the cells whilst preserving the villus-crypt axis in the luminal aspect of the scaffold is also set out. Finally, assessment of produced scaffolds for appropriate characteristics is discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.