ArticleProceedings of the National Academy of Sciences of the United States of America2013
Genome duplication and mutations in ACE2 cause multicellular, fast-sedimenting phenotypes in evolved Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed.
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- Cell division timing shapes the morphology and size of nascent multicellular organisms.bioRxiv : the preprint server for biology · 2026Article
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- Adaptive Laboratory Evolution of Pichia pastoris by Serial Batch Cultivation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Genomic sequencing reveals convergent adaptation during experimental evolution in two budding yeast species.Communications biology · 2024Article
- Optimal trade-off between boosted tolerance and growth fitness during adaptive evolution of yeast to ethanol shocks.Biotechnology for biofuels and bioproducts · 2024Article
- The many faces of Candida auris: Phenotypic and strain variation in an emerging pathogen.PLoS pathogens · 2024Review
- Alternating selection for dispersal and multicellularity favors regulated life cycles.Current biology : CB · 2023Article
- Control of Plant Cell Growth and Proliferation by MO25A, a Conserved Major Component of the Mammalian Sterile 20-Like Kinase Pathway.Plant & cell physiology · 2023Article
- Loss-of-heterozygosity facilitates a fitness valley crossing in experimentally evolved multicellular yeast.Proceedings. Biological sciences · 2022Article
- Overdominant and partially dominant mutations drive clonal adaptation in diploid Saccharomyces cerevisiae.Genetics · 2022Article
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- Adaptation and phenotypic diversification of Bacillus thuringiensis biofilm are accompanied by fuzzy spreader morphotypes.NPJ biofilms and microbiomes · 2022Article
- Drug-dependent growth curve reshaping reveals mechanisms of antifungal resistance in Saccharomyces cerevisiae.Communications biology · 2022Article
- Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals.PLoS biology · 2022Article
- Forward and reverse genetic dissection of morphogenesis identifies filament-competent Candida auris strains.Nature communications · 2021Article
- The genetic basis of differential autodiploidization in evolving yeast populations.G3 (Bethesda, Md.) · 2021Article
- BLAST from the Past: Impacts of Evolving Approaches on Studies of Evolution by Gene Duplication.Genome biology and evolution · 2021Article
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7 authors.
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Abstract
Laboratory evolution of the yeast Saccharomyces cerevisiae in bioreactor batch cultures yielded variants that grow as multicellular, fast-sedimenting clusters. Knowledge of the molecular basis of this phenomenon may contribute to the understanding of natural evolution of multicellularity and to manipulating cell sedimentation in laboratory and industrial applications of S. cerevisiae. Multicellular, fast-sedimenting lineages obtained from a haploid S. cerevisiae strain in two independent evolution experiments were analyzed by whole genome resequencing. The two evolved cell lines showed different frameshift mutations in a stretch of eight adenosines in ACE2, which encodes a transcriptional regulator involved in cell cycle control and mother-daughter cell separation. Introduction of the two ace2 mutant alleles into the haploid parental strain led to slow-sedimenting cell clusters that consisted of just a few cells, thus representing only a partial reconstruction of the evolved phenotype. In addition to single-nucleotide mutations, a whole-genome duplication event had occurred in both evolved multicellular strains. Construction of a diploid reference strain with two mutant ace2 alleles led to complete reconstruction of the multicellular-fast sedimenting phenotype. This study shows that whole-genome duplication and a frameshift mutation in ACE2 are sufficient to generate a fast-sedimenting, multicellular phenotype in S. cerevisiae. The nature of the ace2 mutations and their occurrence in two independent evolution experiments encompassing fewer than 500 generations of selective growth suggest that switching between unicellular and multicellular phenotypes may be relevant for competitiveness of S. cerevisiae in natural environments.
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