ArticleMolecular and cellular biology2014
Microtubule dynamic instability controls podosome patterning in osteoclasts through EB1, cortactin, and Src.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- The Role of Runx2 in Microtubule Dynamics and Its Effects on Osteoblast Migration.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Selective disruption of microtubule formation at the nuclear envelope impairs the bone resorption capacity of osteoclasts.Journal of cell science · 2026Article
- NEK7 phosphorylation of cortactin modulates the migratory capacity of cells expressing EML4-ALK V3.Scientific reports · 2026Article
- Hypergravity reduces F-actin accumulation in osteoclasts, with attenuated bone resorption.PloS one · 2026Article
- In Vitro Cell Culture Model for Osteoclast Activation during Estrogen Withdrawal.International journal of molecular sciences · 2024Article
- α-lipoic acid modulates prostate cancer cell growth and bone cell differentiation.Scientific reports · 2024Article
- Dysfunction in parkin aggravates inflammatory bone erosion by reinforcing osteoclast activity.Cell & bioscience · 2023Article
- The emerging role of microtubules in invasion plasticity.Frontiers in oncology · 2023Review
- Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages.iScience · 2022Article
- Terminally differentiated osteoclasts organize centrosomes into large clusters for microtubule nucleation and bone resorption.Molecular biology of the cell · 2022Article
- Tyrosine Kinase Src Is a Regulatory Factor of Bone Homeostasis.International journal of molecular sciences · 2022Review
- Novel Role of Prereplication Complex Component Cell Division Cycle 6 in Retinal Neovascularization.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Cortactin Contributes to Activity-Dependent Modulation of Spine Actin Dynamics and Spatial Memory Formation.Cells · 2021Article
- Trabecular bone organoids: a micron-scale 'humanised' prototype designed to study the effects of microgravity and degeneration.NPJ microgravity · 2021Article
- Article
- Cortactin function in invadopodia.Small GTPases · 2020Review
- Functional Regeneration of the Sensory Root via Axonal Invasion.Cell reports · 2020Article
- Role of the captured retroviral envelopeBone reports · 2019Article
- TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42.Cell death and differentiation · 2018Article
- S100A4 regulates macrophage invasion by distinct myosin-dependent and myosin-independent mechanisms.Molecular biology of the cell · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
In osteoclasts (OCs) podosomes are organized in a belt, a feature critical for bone resorption. Although microtubules (MTs) promote the formation and stability of the belt, the MT and/or podosome molecules that mediate the interaction of the two systems are not identified. Because the growing "plus" ends of MTs point toward the podosome belt, plus-end tracking proteins (+TIPs) might regulate podosome patterning. Among the +TIPs, EB1 increased as OCs matured and was enriched in the podosome belt, and EB1-positive MTs targeted podosomes. Suppression of MT dynamic instability, displacement of EB1 from MT ends, or EB1 depletion resulted in the loss of the podosome belt. We identified cortactin as an Src-dependent interacting partner of EB1. Cortactin-deficient OCs presented a defective MT targeting to, and patterning of, podosomes and reduced bone resorption. Suppression of MT dynamic instability or EB1 depletion increased cortactin phosphorylation, decreasing its acetylation and affecting its interaction with EB1. Thus, dynamic MTs and podosomes interact to control bone resorption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.