ArticleJournal of cell science2013
Activation of Rac by Asef2 promotes myosin II-dependent contractility to inhibit cell migration on type I collagen.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
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- Copy number variations analysis in a cohort of 47 fetuses and newborns with congenital diaphragmatic hernia.Prenatal diagnosis · 2022Article
- Placental genomics mediates genetic associations with complex health traits and disease.Nature communications · 2022Article
- Parentage testing and looking for single nucleotide markers associated with antler quality in deer (Archives animal breeding · 2022Article
- TheMolecular biology of the cell · 2020Article
- Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma.PLoS genetics · 2020Article
- Cancer-associated fibroblasts promote directional cancer cell migration by aligning fibronectin.The Journal of cell biology · 2017Article
- PTP1B triggers integrin-mediated repression of myosin activity and modulates cell contractility.Biology open · 2015Article
- Non-muscle myosin II in disease: mechanisms and therapeutic opportunities.Disease models & mechanisms · 2015Review
- The guanine nucleotide exchange factor (GEF) Asef2 promotes dendritic spine formation via Rac activation and spinophilin-dependent targeting.The Journal of biological chemistry · 2015Article
- Stretching fibroblasts remodels fibronectin and alters cancer cell migration.Scientific reports · 2015Article
- Automated analysis of cell-matrix adhesions in 2D and 3D environments.Scientific reports · 2015Article
- TRPM4 Is a Novel Component of the Adhesome Required for Focal Adhesion Disassembly, Migration and Contractility.PloS one · 2015Article
- Phosphorylation of serine 106 in Asef2 regulates cell migration and adhesion turnover.Journal of proteome research · 2014Article
- The Rho family GEF Asef2 regulates cell migration in three dimensional (3D) collagen matrices through myosin II.Cell adhesion & migration · 2014Article
- I'm coming to GEF you: Regulation of RhoGEFs during cell migration.Cell adhesion & migration · 2014Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Non-muscle myosin II (MyoII) contractility is central to the regulation of numerous cellular processes, including migration. Rho is a well-characterized modulator of actomyosin contractility, but the function of other GTPases, such as Rac, in regulating contractility is currently not well understood. Here, we show that activation of Rac by the guanine nucleotide exchange factor Asef2 (also known as SPATA13) impairs migration on type I collagen through a MyoII-dependent mechanism that enhances contractility. Knockdown of endogenous Rac or treatment of cells with a Rac-specific inhibitor decreases the amount of active MyoII, as determined by serine 19 (S19) phosphorylation, and negates the Asef2-promoted increase in contractility. Moreover, treatment of cells with blebbistatin, which inhibits MyoII activity, abolishes the Asef2-mediated effect on migration. In addition, Asef2 slows the turnover of adhesions in protrusive regions of cells by promoting large mature adhesions, which has been linked to actomyosin contractility, with increased amounts of active β1 integrin. Hence, our data reveal a new role for Rac activation, promoted by Asef2, in modulating actomyosin contractility, which is important for regulating cell migration and adhesion dynamics.
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Registered trials
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