ArticleBiosensors & bioelectronics2014
On-line protein capture on magnetic beads for ultrasensitive microfluidic immunoassays of cancer biomarkers.
Article in Biosensors & bioelectronics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 118 citations in OpenAlex.
- Emerging roles of microfluidics in oral cancer research and clinical translation.Materials today. Bio · 2026Review
- Molecular Insights into the Engagement of High-Affinity Sialylated Ligands to Human CD22.JACS Au · 2025Article
- Review
- Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment.Mechanobiology in medicine · 2024Review
- Photoactive hydrogels for pre-concentration, labelling, and controlled release of proteins.The Analyst · 2023Article
- Mapping the Potential of Microfluidics in Early Diagnosis and Personalized Treatment of Head and Neck Cancers.Cancers · 2023Review
- Multiplexed electrochemical assays for clinical applications.Current opinion in electrochemistry · 2023Article
- MoSDiagnostics (Basel, Switzerland) · 2023Article
- Tunable Magneto-Plasmonic Nanosensor for Sensitive Detection of Foodborne Pathogens.Biosensors · 2023Article
- Review
- Sandwich Fluorescence Detection of Foodborne PathogenFoods (Basel, Switzerland) · 2022Article
- A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers.Analytical and bioanalytical chemistry · 2022Article
- Multiplexed Prostate Cancer Companion Diagnostic Devices.Sensors (Basel, Switzerland) · 2021Review
- Recent Advances in Nano-Bio-Sensing Fabrication Technology for the Detection of Oral Cancer.Molecular biotechnology · 2021Review
- Review
- Sub-zeptomole Detection of Biomarker Proteins Using a Microfluidic Immunoarray with Nanostructured Sensors.Analytical chemistry · 2020Article
- Signal amplification in immunoassays by using noble metal nanoparticles: a review.Mikrochimica acta · 2019Review
- Microfluidic Magnetic Mixing at Low Reynolds Numbers and in Stagnant Fluids.Micromachines · 2019Review
- Screen-Printed Soft-Nitrided Carbon Electrodes for Detection of Hydrogen Peroxide.Sensors (Basel, Switzerland) · 2019Article
- Molecular profiling of tissue biopsies reveals unique signatures associated with streptococcal necrotizing soft tissue infections.Nature communications · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 4 countries.
Funding
Abstract
Accurate, sensitive, multiplexed detection of biomarker proteins holds significant promise for personalized cancer diagnostics. Here we describe the incorporation of a novel on-line chamber to capture cancer biomarker proteins on magnetic beads derivatized with 300,000 enzyme labels and 40,000 antibodies into a modular microfluidic immunoarray. Capture and detection chambers are produced from PDMS on machined molds and do not require lithography. Protein analytes are captured from serum or other biological samples in the stirred capture chamber on the beads held in place magnetically. The beads are subsequently washed free of sample components, and wash solutions sent to waste. Removal of the magnet and valve switching sends the magnetic bead-protein bioconjugates into a detection chamber where they are captured on 8 antibody-decorated gold nanoparticle-film sensors and detected amperometrically. Most steps in the immunoassay including protein capture, washing and measurement are incorporated into the device. In simultaneous assays, the microfluidic system gave ultralow detection limits of 5 fg mL(-1) for interleukin-6 (IL-6) and 7 fg mL(-1) for IL-8 in serum. Accuracy was demonstrated by measuring IL-6 and IL-8 in conditioned media from oral cancer cell lines and showing good correlations with standard ELISAs. The on-line capture chamber facilitates rapid, sensitive, repetitive protein separation and measurement in 30 min in a semi-automated system adaptable to multiplexed protein detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.