ArticleAnnals of clinical microbiology and antimicrobials2013
The establishment of a duplex real-time PCR assay for rapid and simultaneous detection of blaNDM and blaKPC genes in bacteria.
Article in Annals of clinical microbiology and antimicrobials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Visual Detection for Environmental Antimicrobial Resistance Genes by Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick.Current microbiology · 2026Article
- A Multiplex Recombinase-Aided qPCR Assay for Highly Sensitive and Rapid Detection of khe, blaJournal of clinical laboratory analysis · 2024Article
- Rapid On-Site Detection of Extensively Drug-Resistant Genes inMicrobiology spectrum · 2022Article
- Antimicrobial resistance-Do we share more than companionship with our dogs?Journal of applied microbiology · 2022Article
- Rapid detection of a novel B1-β-lactamase gene, blaAFM-1 using a loop-mediated isothermal amplification (LAMP) assay.Annals of clinical microbiology and antimicrobials · 2021Article
- Validation of specific quantitative real-time RT-PCR assay panel for Infectious Bronchitis using synthetic DNA standards and clinical specimens.Journal of virological methods · 2020Article
- Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis.BMC microbiology · 2019Article
- NDM Metallo-β-Lactamases and Their Bacterial Producers in Health Care Settings.Clinical microbiology reviews · 2019Review
- Development of a Multiplex PCR Platform for the Rapid Detection of Bacteria, Antibiotic Resistance, andFrontiers in cellular and infection microbiology · 2019Article
- Appropriateness of empiric antimicrobial therapy with imipenem/colistin in severe septic patients: observational cohort study.Annals of clinical microbiology and antimicrobials · 2018Observational
- Comparison of in-house and commercial real time-PCR based carbapenemase gene detection methods in Enterobacteriaceae and non-fermenting gram-negative bacterial isolates.Annals of clinical microbiology and antimicrobials · 2017Article
- Evaluation of the Check-Points Check MDR CT103 and CT103 XL Microarray Kits by Use of Preparatory Rapid Cell Lysis.Journal of clinical microbiology · 2016Article
- Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples.Journal of clinical microbiology · 2015Article
- Assessment of combination therapy in BALB/c mice injected with carbapenem-resistant Enterobacteriaceae strains.Frontiers in microbiology · 2015Article
- Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management.BioMed research international · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase New Delhi metallo-β-lactamase (NDM) and Klebsiella pneumoniae carbapenemase (KPC) producers. Rapid molecular detection is essential to limit their spread. In this study, a duplex real-time polymerase chain reaction (PCR) that was specific for the detection of blaNDM and blaKPC with the same limit of detection of ten plasmid copies was developed. The assay was linear over eight log dilutions for blaNDM (R2 = 0.971; slope, -3.273) and blaKPC (R2 = 0.992; slope, -2.997) with efficiencies of 102% and 115%, respectively. The assay was validated with 157 clinical isolates and showed 100% concordance with conventional PCR. The excellent performance of the duplex PCR assay makes it a powerful tool for surveillance of the carbapenemases NDM and KPC.
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