ArticlePloS one2013
MADM-ML, a mouse genetic mosaic system with increased clonal efficiency.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 36 citations in OpenAlex.
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- Orderly specification and precise laminar deployment of mouse cortical projection neuron types through intermediate progenitors.Developmental cell · 2025Article
- Human embryonic genetic mosaicism and its effects on development and disease.Nature reviews. Genetics · 2024Review
- Toxoplasma gondii infection supports the infiltration of T cells into brain tumors.Journal of neuroimmunology · 2024Article
- A genetic mosaic mouse model illuminates the pre-malignant progression of basal-like breast cancer.Disease models & mechanisms · 2023Article
- Systemic Hypoxemia Induces Cardiomyocyte Hypertrophy and Right Ventricular Specific Induction of Proliferation.Circulation research · 2023Article
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- In Vivo Methods to Monitor Cardiomyocyte Proliferation.Journal of cardiovascular development and disease · 2022Review
- Multicolor strategies for investigating clonal expansion and tissue plasticity.Cellular and molecular life sciences : CMLS · 2022Review
- Dual recombinases-based genetic lineage tracing for stem cell research with enhanced precision.Science China. Life sciences · 2021Review
- Deep neural net tracking of human pluripotent stem cells reveals intrinsic behaviors directing morphogenesis.Stem cell reports · 2021Article
- Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes.PLoS biology · 2021Article
- Genetic lineage tracing with multiple DNA recombinases: A user's guide for conducting more precise cell fate mapping studies.The Journal of biological chemistry · 2020Review
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- Linking neuronal lineage and wiring specificity.Neural development · 2018Review
- Echoes of the embryo: using the developmental biology toolkit to study cancer.Disease models & mechanisms · 2016Review
- Corneal endothelial cells possess an elaborate multipolar shape to maximize the basolateral to apical membrane area.Molecular vision · 2016Article
- Cardiac Regeneration and Stem Cells.Physiological reviews · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mosaic Analysis with Double Markers (MADM) is a mouse genetic system that allows simultaneous gene knockout and fluorescent labeling of sparse, clonally-related cells within an otherwise normal mouse, thereby circumventing embryonic lethality problems and providing single-cell resolution for phenotypic analysis in vivo. The clonal efficiency of MADM is intrinsically low because it relies on Cre/loxP-mediated mitotic recombination between two homologous chromosomes rather than within the same chromosome, as in the case of conditional knockout (CKO). Although sparse labeling enhances in vivo resolution, the original MADM labels too few or even no cells when a low-expressing Cre transgene is used or a small population of cells is studied. Recently, we described the usage of a new system, MADM-ML, which contains three mutually exclusive, self-recognizing loxP variant sites as opposed to a single loxP site present in the original MADM system (referred to as MADM-SL in this paper). Here we carefully compared the recombination efficiency between MADM-SL and MADM-ML using the same Cre transgene, and found that the new system labels significantly more cells than the original system does. When we established mouse medulloblastoma models with both the original and the new MADM systems, we found that, while the MADM-SL model suffered from varied tumor progression and incomplete penetrance, the MADM-ML model had consistent tumor progression and full penetrance of tumor formation. Therefore MADM-ML, with its higher recombination efficiency, will broaden the applicability of MADM for studying many biological questions including normal development and disease modeling at cellular resolution in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.