ArticlePloS one2013
High-throughput sequencing reveals differential expression of miRNAs in intestine from sea cucumber during aestivation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 72 citations in OpenAlex.
- Transcriptome Analysis Reveals the lncRNA-mRNA Co-expression Network Regulating the Aestivation of Sea Cucumber.Marine biotechnology (New York, N.Y.) · 2024Article
- Aestivation induces widespread transcriptional changes in the African lungfish.Frontiers in genetics · 2023Article
- MiRNA-mRNA Integration Analysis Reveals the Regulatory Roles of MiRNAs in Shell Pigmentation of the Manila clam (Ruditapes philippinarum).Marine biotechnology (New York, N.Y.) · 2021Article
- MicroRNA regulation in hypoxic environments: differential expression of microRNAs in the liver of largemouth bass (Micropterus salmoides).Fish physiology and biochemistry · 2020Article
- MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation inCells · 2019Article
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- Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (G3 (Bethesda, Md.) · 2017Article
- Evidence for a Saponin Biosynthesis Pathway in the Body Wall of the Commercially Significant Sea Cucumber Holothuria scabra.Marine drugs · 2017Article
- Differential Expression of miRNAs in the Respiratory Tree of the Sea CucumberG3 (Bethesda, Md.) · 2017Article
- Long Non-Coding RNAs (lncRNAs) of Sea Cucumber: Large-Scale Prediction, Expression Profiling, Non-Coding Network Construction, and lncRNA-microRNA-Gene Interaction Analysis of lncRNAs in Apostichopus japonicus and Holothuria glaberrima During LPS Challenge and Radial Organ Complex Regeneration.Marine biotechnology (New York, N.Y.) · 2016Article
- The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns.Scientific reports · 2016Article
- High throughput sequencing of small RNAs transcriptomes in two Crassostrea oysters identifies microRNAs involved in osmotic stress response.Scientific reports · 2016Article
- Comparative transcriptome analysis of papilla and skin in the sea cucumber, Apostichopus japonicus.PeerJ · 2016Article
- Identification and profiling of miRNAs in the freeze-avoiding gall moth Epiblema scudderiana via next-generation sequencing.Molecular and cellular biochemistry · 2015Article
- Bias in microRNA functional enrichment analysis.Bioinformatics (Oxford, England) · 2015Article
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- Down but Not Out: The Role of MicroRNAs in Hibernating Bats.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulatory role of miRNA in gene expression is an emerging hot new topic in the control of hypometabolism. Sea cucumber aestivation is a complicated physiological process that includes obvious hypometabolism as evidenced by a decrease in the rates of oxygen consumption and ammonia nitrogen excretion, as well as a serious degeneration of the intestine into a very tiny filament. To determine whether miRNAs play regulatory roles in this process, the present study analyzed profiles of miRNA expression in the intestine of the sea cucumber (Apostichopus japonicus), using Solexa deep sequencing technology. We identified 308 sea cucumber miRNAs, including 18 novel miRNAs specific to sea cucumber. Animals sampled during deep aestivation (DA) after at least 15 days of continuous torpor, were compared with animals from a non-aestivation (NA) state (animals that had passed through aestivation and returned to the active state). We identified 42 differentially expressed miRNAs [RPM (reads per million) >10, |FC| (|fold change|) ≥ 1, FDR (false discovery rate) <0.01] during aestivation, which were validated by two other miRNA profiling methods: miRNA microarray and real-time PCR. Among the most prominent miRNA species, miR-200-3p, miR-2004, miR-2010, miR-22, miR-252a, miR-252a-3p and miR-92 were significantly over-expressed during deep aestivation compared with non-aestivation animals. Preliminary analyses of their putative target genes and GO analysis suggest that these miRNAs could play important roles in global transcriptional depression and cell differentiation during aestivation. High-throughput sequencing data and microarray data have been submitted to GEO database.
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