ArticleG3 (Bethesda, Md.)2013
Regulation of gene expression in Neurospora crassa with a copper responsive promoter.
Article in G3 (Bethesda, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Xy-31 and Lat-1 regulate the induction of polysaccharide disintegrating genes by arabinose inFrontiers in microbiology · 2026Article
- Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum.BMC biotechnology · 2023Article
- Article
- Development of a versatile copper-responsive gene expression system in the plant-pathogenic fungus Fusarium graminearum.Molecular plant pathology · 2021Article
- Chromatin accessibility profiling in Neurospora crassa reveals molecular features associated with accessible and inaccessible chromatin.BMC genomics · 2021Article
- Article
- Shannon entropy as a metric for conditional gene expression in Neurospora crassa.G3 (Bethesda, Md.) · 2021Article
- Circadian clock control of eIF2α phosphorylation is necessary for rhythmic translation initiation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Circadian clock regulation of the glycogen synthase (Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Light-regulated promoters for tunable, temporal, and affordable control of fungal gene expression.Applied microbiology and biotechnology · 2018Review
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Abnormal Ergosterol Biosynthesis Activates Transcriptional Responses to Antifungal Azoles.Frontiers in microbiology · 2018Article
- The Neurospora Transcription Factor ADV-1 Transduces Light Signals and Temporal Information to Control Rhythmic Expression of Genes Involved in Cell Fusion.G3 (Bethesda, Md.) · 2017Article
- Accumulation of specific sterol precursors targets a MAP kinase cascade mediating cell-cell recognition and fusion.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Calcineurin Subunits A and B Interact to Regulate Growth and Asexual and Sexual Development in Neurospora crassa.PloS one · 2016Article
- A Tool Set for the Genome-Wide Analysis of Neurospora crassa by RT-PCR.G3 (Bethesda, Md.) · 2015Article
- Metabolic Impacts of Using Nitrogen and Copper-Regulated Promoters to Regulate Gene Expression in Neurospora crassa.G3 (Bethesda, Md.) · 2015Article
- Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei.Biotechnology for biofuels · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled by copper availability. Excess copper represses, and copper depletion, via the use of a copper chelator, activates expression. The kinetics of induction and repression of tcu-1 are rapid, and the effects are long lived. We constructed a plasmid carrying the bar gene (for glufosinate selection) fused to the tcu-1 promoter. This plasmid permits the generation of DNA fragments that can direct integration of Ptcu-1 into any desired locus. We use this strategy to integrate Ptcu-1 in front of wc-1, a circadian oscillator and photoreceptor gene. The addition of excess copper to the Ptcu-1::wc-1 strain phenocopies a Δwc-1 strain, and the addition of the copper chelator, bathocuproinedisulfonic acid, phenocopies a wc-1 overexpression strain. To test whether copper repression can recapitulate the loss of viability that an essential gene knockout causes, we placed Ptcu-1 upstream of the essential gene, hpt-1. The addition of excess copper drastically reduced the growth rate as expected. Thus, this strategy will be useful to probe the biological function of any N. crassa gene through controlled expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.