ArticleAmerican journal of physiology. Lung cellular and molecular physiology2014
Methemoglobin-induced signaling and chemokine responses in human alveolar epithelial cells.
Article in American journal of physiology. Lung cellular and molecular physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Advances in acute respiratory distress syndrome: focusing on heterogeneity, pathophysiology, and therapeutic strategies.Signal transduction and targeted therapy · 2025Review
- Cell-Free Hemoglobin in the Pathophysiology of Trauma: A Scoping Review.Critical care explorations · 2024Article
- Cell-free hemoglobin triggers macrophage cytokine production via TLR4 and MyD88.American journal of physiology. Lung cellular and molecular physiology · 2024Article
- Whole-blood ribonucleic acid sequencing analysis in methemoglobinemia: a case report.Journal of medical case reports · 2023Article
- Ultraviolet light oxidation of fresh hemoglobin eliminates aggregate formation seen in commercially sourced hemoglobin.Blood cells, molecules & diseases · 2023Article
- SARS-CoV-2 Proteins Bind to Hemoglobin and Its Metabolites.International journal of molecular sciences · 2021Article
- The Role of Methemoglobin and Carboxyhemoglobin in COVID-19: A Review.Journal of clinical medicine · 2020Review
- The NLRP3 inflammasome in macrophages is stimulated by cell-free hemoglobin.Physiological reports · 2020Article
- The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells.Scientific reports · 2018Article
- Purified and Recombinant Hemopexin: Protease Activity and Effect on Neutrophil Chemotaxis.Molecular medicine (Cambridge, Mass.) · 2016Article
- Oxidized Ferric and Ferryl Forms of Hemoglobin Trigger Mitochondrial Dysfunction and Injury in Alveolar Type I Cells.American journal of respiratory cell and molecular biology · 2016Article
- Cell-free hemoglobin: a novel mediator of acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- A Pathophysiologic Approach to Biomarkers in Acute Respiratory Distress Syndrome.Disease markers · 2016Review
- Haptoglobin attenuates hemoglobin-induced heme oxygenase-1 in renal proximal tubule cells and kidneys of a mouse model of sickle cell disease.Blood cells, molecules & diseases · 2015Article
- Fanning the Fire: Can Methemoglobin Enhance Neutrophil Activation?EBioMedicine · 2015Article
- The role of red blood cells and cell-free hemoglobin in the pathogenesis of ARDS.Journal of intensive care · 2015Review
- Thinking outside the cell: how cell-free hemoglobin can potentiate acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2014Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Diffuse alveolar hemorrhage is characterized by the presence of red blood cells and free hemoglobin in the alveoli and complicates a number of serious medical and surgical lung conditions including the pulmonary vasculitides and acute respiratory distress syndrome. In this study we investigated the hypothesis that exposure of human alveolar epithelial cells to hemoglobin and its breakdown products regulates chemokine release via iron- and oxidant-mediated activation of the transcription factor NF-κB. Methemoglobin alone stimulated the release of IL-8 and MCP-1 from A549 cells via activation of the NF-κB pathway; additionally, IL-8 required ERK activation and MCP-1 required JNK activation. Neither antioxidants nor iron chelators and knockdown of ferritin heavy and light chains affected these responses, indicating that iron and reactive oxygen species are not involved in the response of alveolar epithelial cells to methemoglobin. Incubation of primary cultures of human alveolar type 2 cells with methemoglobin resulted in a similar pattern of chemokine release and signaling pathway activation. In summary, we have shown for the first time that methemoglobin induced chemokine release from human lung epithelial cells independent of iron- and redox-mediated signaling involving the activation of the NF-κB and MAPK pathways. Decompartmentalization of hemoglobin may be a significant proinflammatory stimulus in a variety of lung diseases.
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