ArticleRNA (New York, N.Y.)2013
Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7).
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Recent Advances in Chemical Probing Strategies for RNA Structure Determination In Vivo.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Mapping the RNA Chaperone Activity of the Trypanosoma brucei Editosome Using SHAPE-Chemical Probing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A systematic search for RNA structural switches across the human transcriptome.Nature methods · 2024Article
- Modulation of pre-mRNA structure by hnRNP proteins regulates alternative splicing ofScience advances · 2022Article
- Retron reverse transcriptase termination and phage defense are dependent on host RNase H1.Nucleic acids research · 2022Article
- Folding heterogeneity in the essential human telomerase RNA three-way junction.RNA (New York, N.Y.) · 2020Article
- An evolutionarily conserved RNA structure in the functional core of the lincRNA Cyrano.RNA (New York, N.Y.) · 2020Article
- The structure of the influenza A virus genome.Nature microbiology · 2019Article
- In-cell RNA structure probing with SHAPE-MaP.Nature protocols · 2018Article
- SHAPE analysis of theRNA (New York, N.Y.) · 2017Article
- High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast.Nature communications · 2017Article
- Kaposi's sarcoma-associated herpesvirus polyadenylated nuclear RNA: a structural scaffold for nuclear, cytoplasmic and viral proteins.Nucleic acids research · 2017Article
- The 2D Structure of theJournal of nucleic acids · 2017Article
- Characterizing RNA structures in vitro and in vivo with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq).Methods (San Diego, Calif.) · 2016Article
- The Functional Role of eL19 and eB12 Intersubunit Bridge in the Eukaryotic Ribosome.Journal of molecular biology · 2016Article
- The RNA chaperone activity of the Trypanosoma brucei editosome raises the dynamic of bound pre-mRNAs.Scientific reports · 2016Article
- Selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurate RNA structure analysis.Nature protocols · 2015Article
- A Faster Triphosphorylation Ribozyme.PloS one · 2015Article
- Secondary structure of the Irf7 5'-UTR, analyzed using SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension).BMB reports · 2014Article
- A ribozyme that triphosphorylates RNA 5'-hydroxyl groups.Nucleic acids research · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Estimating the reactivity of 2'-hydroxyl groups along an RNA chain of interest aids in the modeling of the folded RNA structure; flexible loops tend to be reactive, whereas duplex regions are generally not. Among the most useful reagents for probing 2'-hydroxyl reactivity is 1-methyl-7-nitroisatoic anhydride (1m7), but the absence of a reliable, inexpensive source has prevented widespread adoption. An existing protocol for the conversion of an inexpensive precursor 4-nitroisatoic anhydride (4NIA) recommends the use of NaH in dimethylformamide (DMF), a reagent combination that most molecular biology labs are not equipped to handle, and that does not scale safely in any case. Here we describe a safer, one-pot method for bulk conversion of 4NIA to 1m7 that reduces costs and bypasses the use of NaH. We show that 1m7 produced by this method is free of side products and can be used to probe RNA structure in vitro.
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