ArticleMolecules (Basel, Switzerland)2013
SOCS3-mediated blockade reveals major contribution of JAK2/STAT5 signaling pathway to lactation and proliferation of dairy cow mammary epithelial cells in vitro.
Article in Molecules (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Differential gene regulatory network analysis reveals transcriptional disruption in opioid.NAR genomics and bioinformatics · 2026Article
- ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.Animal bioscience · 2026Article
- Molecular Characteristics ofInternational journal of molecular sciences · 2024Article
- A common regulatory haplotype doubles lactoferrin concentration in milk.Genetics, selection, evolution : GSE · 2024Article
- Identifying differentially expressed genes in goat mammary epithelial cells induced by overexpression ofFrontiers in veterinary science · 2024Article
- D-Methionine and 2-hydroxy-4-methylthiobutanoic acid i alter beta-casein, proteins and metabolites linked in milk protein synthesis in bovine mammary epithelial cells.Journal of animal science and technology · 2022Article
- Mutation of Signal Transducer and Activator of Transcription 5 (STAT5) Binding Sites Decreases Milk Allergen αFoods (Basel, Switzerland) · 2022Article
- Article
- Hepatic transcriptome analysis identifies genes, polymorphisms and pathways involved in the fatty acids metabolism in sheep.PloS one · 2021Article
- Article
- Role of the JAK-STAT Pathway in Bovine Mastitis and Milk Production.Animals : an open access journal from MDPI · 2020Review
- Protective effects of valsartan administration on doxorubicin‑induced myocardial injury in rats and the role of oxidative stress and NOX2/NOX4 signaling.Molecular medicine reports · 2020Article
- Overview of Folic Acid Supplementation Alone or in Combination with Vitamin B12 in Dairy Cattle during Periparturient Period.Metabolites · 2020Review
- DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2018Article
- Kisspeptin-10 Induces β-Casein Synthesis via GPR54 and Its Downstream Signaling Pathways in Bovine Mammary Epithelial Cells.International journal of molecular sciences · 2017Article
- Syringaresinol-4-Acta pharmaceutica Sinica. B · 2017Article
- Biological underpinnings of breastfeeding challenges: the role of genetics, diet, and environment on lactation physiology.American journal of physiology. Endocrinology and metabolism · 2016Review
- Aryl Hydrocarbon Receptor Plays Protective Roles against High Fat Diet (HFD)-induced Hepatic Steatosis and the Subsequent Lipotoxicity via Direct Transcriptional Regulation of Socs3 Gene Expression.The Journal of biological chemistry · 2016Article
- Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells.International journal of molecular sciences · 2015Article
- 14-3-3γ affects mTOR pathway and regulates lactogenesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2015Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Suppressor of cytokine signaling 3 (SOCS3) is a cytokine-induced negative feedback-loop regulator of cytokine signaling. More and more evidence has proved it to be an inhibitor of signal transducers and activators of transcription 5 (STAT5). Here, we used dairy cow mammary epithelial cells (DCMECs) to analyze the function of SOCS3 and the interaction between SOCS3 and STAT5a. The expression of SOCS3 was found in cytoplasm and nucleus of DCMECs by fluorescent immunostaining. Overexpression and inhibition of SOCS3 brought a remarkable milk protein synthesis change through the regulation of JAK2/STAT5a pathway activity, and SOCS3 expression also decreased SREBP-1c expression and fatty acid synthesis. Inhibited STAT5a activation correlated with reduced SOCS3 expression, which indicated that SOCS3 gene might be one of the targets of STAT5a activation, DCMECs treated with L-methionine (Met) resulted in a decrease of SOCS3 expression. SOCS3 could also decrease cell proliferation and viability by CASY-TT detection. Together, our findings indicate that SOCS3 acts as an inhibitor of JAK2/STAT5a pathway and disturbs fatty acid synthesis by decreasing SREBP-1c expression, which validates its involvement in both milk protein synthesis and fat synthesis. In aggregate, these results reveal that low SOCS3 expression is required for milk synthesis and proliferation of DCMECs in vitro.
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