ArticleApplied and environmental microbiology2014
Estimating the prevalence of potential enteropathogenic Escherichia coli and intimin gene diversity in a human community by monitoring sanitary sewage.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Machine learning-enabled wastewater-based surveillance for emerging pathogen detection and monitoring: current applications, challenges, and future prospects.Emerging microbes & infections · 2026Review
- Wastewater-based Surveillance ofChina CDC weekly · 2025Article
- Development of a wastewater based infectious disease surveillance research system in South Korea.Scientific reports · 2024Article
- Molecular Markers and Antimicrobial Resistance Patterns of Extraintestinal PathogenicTropical medicine and infectious disease · 2024Article
- Wastewater treatment plants, an "escape gate" for ESCAPE pathogens.Frontiers in microbiology · 2023Review
- Sulforaphane Ameliorates the Intestinal Injury in Necrotizing Enterocolitis by Regulating the PI3K/Akt/GSK-3Canadian journal of gastroenterology & hepatology · 2022Article
- Comparative genomics of Chinese and international isolates ofMicrobial genomics · 2021Article
- Technical framework for wastewater-based epidemiology of SARS-CoV-2.The Science of the total environment · 2021Article
- Fine-Scale Temporal Dynamics of SARS-CoV-2 RNA Abundance in Wastewater during A COVID-19 Lockdown.Water research · 2021Article
- Municipal Wastewater Surveillance Revealed a High Community Disease Burden of a Rarely Reported and Possibly SubclinicalApplied and environmental microbiology · 2020Article
- Characterization ofPathogens (Basel, Switzerland) · 2020Article
- Repeated Isolation of Extended-Spectrum-β-Lactamase-PositiveAntimicrobial agents and chemotherapy · 2019Article
- Change in the Structure ofFrontiers in microbiology · 2017Article
- Detection of pathogenic Campylobacter, E. coli O157:H7 and Salmonella spp. in wastewater by PCR assay.Environmental science and pollution research international · 2016Article
- Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments.Applied and environmental microbiology · 2016Article
- Genetic Diversity of Intimin Gene of Atypical Enteropathogenic Escherichia coli Isolated from Human, Animals and Raw Meats in China.PloS one · 2016Article
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3 authors.
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Abstract
Presently, the understanding of bacterial enteric diseases in the community and their virulence factors relies almost exclusively on clinical disease reporting and examination of clinical pathogen isolates. This study aimed to investigate the feasibility of an alternative approach that monitors potential enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) prevalence and intimin gene (eae) diversity in a community by directly quantifying and characterizing target virulence genes in the sanitary sewage. The quantitative PCR (qPCR) quantification of the eae, stx1, and stx2 genes in sanitary sewage samples collected over a 13-month period detected eae in all 13 monthly sewage samples at significantly higher abundance (93 to 7,240 calibrator cell equivalents [CCE]/100 ml) than stx1 and stx2, which were detected sporadically. The prevalence level of potential EPEC in the sanitary sewage was estimated by calculating the ratio of eae to uidA, which averaged 1.0% (σ = 0.4%) over the 13-month period. Cloning and sequencing of the eae gene directly from the sewage samples covered the majority of the eae diversity in the sewage and detected 17 unique eae alleles belonging to 14 subtypes. Among them, eae-β2 was identified to be the most prevalent subtype in the sewage, with the highest detection frequency in the clone libraries (41.2%) and within the different sampling months (85.7%). Additionally, sewage and environmental E. coli isolates were also obtained and used to determine the detection frequencies of the virulence genes as well as eae genetic diversity for comparison.
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