ArticleApplied and environmental microbiology2014
Single-cell analyses revealed transfer ranges of IncP-1, IncP-7, and IncP-9 plasmids in a soil bacterial community.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 76 citations in OpenAlex.
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- Transconjugant range of PromA plasmids in microbial communities is predicted by sequence similarity with the bacterial host chromosome.Microbial genomics · 2023Article
- Validation of the application of gel beads-based single-cell genome sequencing platform to soil and seawater.ISME communications · 2022Article
- Hitherto-Unnoticed Self-Transmissible Plasmids Widely Distributed among Different Environments in Japan.Applied and environmental microbiology · 2022Article
- The impact of pollutant as selection pressure on conjugative transfer of dioxin-catabolic plasmids harbored by Rhodococcus sp. strain p52.Environmental science and pollution research international · 2022Article
- Fluviispira sanaruensis sp., nov., Isolated from a Brackish Lake in Hamamatsu, Japan.Current microbiology · 2021Article
- Effects of monochloramine on culturability, viability and persistence of Pseudomonas putida and tap water mixed bacterial community.Applied microbiology and biotechnology · 2021Article
- Bioremediation byMicroorganisms · 2020Article
- Multiple plasmid origin-of-transfer regions might aid the spread of antimicrobial resistance to human pathogens.MicrobiologyOpen · 2020Article
- Zinc can counteract selection for ciprofloxacin resistance.FEMS microbiology letters · 2020Article
- Determination of Plasmid pSN1216-29 Host Range and the Similarity in Oligonucleotide Composition Between Plasmid and Host Chromosomes.Frontiers in microbiology · 2020Article
- Plant Species-Dependent Increased Abundance and Diversity of IncP-1 Plasmids in the Rhizosphere: New Insights Into Their Role and Ecology.Frontiers in microbiology · 2020Article
- Selection for antimicrobial resistance is reduced when embedded in a natural microbial community.The ISME journal · 2019Article
- A Human Lung-AssociatedFrontiers in microbiology · 2019Article
- Fluorescence Recovery Allows the Implementation of a Fluorescence Reporter Gene Platform Applicable for the Detection and Quantification of Horizontal Gene Transfer in Anoxic Environments.Applied and environmental microbiology · 2018Article
- Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.Frontiers in microbiology · 2018Article
- Different transferability of incompatibility (Inc) P-7 plasmid pCAR1 and IncP-1 plasmid pBP136 in stirring liquid conditions.PloS one · 2017Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conjugative transfer ranges of three different plasmids of the incompatibility groups IncP-1 (pBP136), IncP-7 (pCAR1), and IncP-9 (NAH7) were investigated in soil bacterial communities by culture-dependent and culture-independent methods. Pseudomonas putida, a donor of each plasmid, was mated with soil bacteria, and green fluorescent protein (GFP), encoded on the plasmid, was used as a reporter protein for successful transfer. GFP-expressing transconjugants were detected and separated at the single-cell level by flow cytometry. Each cell was then analyzed by PCR and sequencing of its 16S rRNA gene following either whole-genome amplification or cultivation. A large number of bacteria within the phylum Proteobacteria was identified as transconjugants for pBP136 by both culture-dependent and culture-independent methods. Transconjugants belonging to the phyla Actinobacteria, Bacteroidetes, and Firmicutes were detected only by the culture-independent method. Members of the genus Pseudomonas (class Gammaproteobacteria) were identified as major transconjugants of pCAR1 and NAH7 by both methods, whereas Delftia species (class Betaproteobacteria) were detected only by the culture-independent method. The transconjugants represented a minority of the soil bacteria. Although pCAR1-containing Delftia strains could not be cultivated after a one-to-one filter mating assay between the donor and cultivable Delftia strains as recipients, fluorescence in situ hybridization detected pCAR1-containing Delftia cells, suggesting that Delftia was a "transient" host of pCAR1.
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