ArticleAnalytical biochemistry2014
Refining membrane penetration by a combination of steady-state and time-resolved depth-dependent fluorescence quenching.
Article in Analytical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Binding orientation of weakly associating membrane peripheral proteins via membrane paramagnetic relaxation enhancement NMR.Communications chemistry · 2026Article
- Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein-Lipid Interactions.Chemical record (New York, N.Y.) · 2024Review
- Determination of the boundary lipids of sticholysins using tryptophan quenching.Scientific reports · 2022Article
- Location of TEMPO-PC in Lipid Bilayers: Implications for Fluorescence Quenching.The Journal of membrane biology · 2020Article
- Refining Protein Penetration into the Lipid Bilayer Using Fluorescence Quenching and Molecular Dynamics Simulations: The Case of Diphtheria Toxin Translocation Domain.The Journal of membrane biology · 2018Article
- Location of the TEMPO Moiety of TEMPO-PC in Lipid Bilayers.Biophysical journal · 2017Article
- Effects of Dimethyl Sulfoxide on Surface Water near Phospholipid Bilayers.Biophysical journal · 2016Article
- Calibration of Distribution Analysis of the Depth of Membrane Penetration Using Simulations and Depth-Dependent Fluorescence Quenching.The Journal of membrane biology · 2015Article
- Measuring membrane penetration with depth-dependent fluorescence quenching: distribution analysis is coming of age.Biochimica et biophysica acta · 2014Review
- Structural plasticity in the topology of the membrane-interacting domain of HIV-1 gp41.Biophysical journal · 2014Article
- Lipid-modulation of membrane insertion and refolding of the apoptotic inhibitor Bcl-xL.Biochimica et biophysica acta. Proteins and proteomicsArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Accurate determination of the depth of membrane penetration of a fluorescent probe, attached to a lipid, protein, or other macromolecule of interest, using depth-dependent quenching methodology is complicated by thermal motion in the lipid bilayer. Here, we suggest that a combination of steady-state and time-resolved measurements can be used to generate a static quenching profile that reduces the contribution from transverse diffusion occurring during the excited-state lifetime. This procedure results in narrower quenching profiles, compared with those obtained by traditional measurements, and thus improves precision in determination of the underlying depth distribution of the probe.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.