ArticleCurrent biology : CB2013
Live imaging of bicoid-dependent transcription in Drosophila embryos.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed.
- Dynamics of bicoid mRNA localisation and translation dictate morphogen gradient formation.The EMBO journal · 2026Article
- Single-cell transcriptional dynamics in a living vertebrate.Cell systems · 2026Article
- Decoupling between activation time and steady-state level in input-output responses.PLoS computational biology · 2026Article
- Identification of optimal fluorophores for use in theMolecular biology of the cell · 2026Article
- Machine Learning-Driven Prediction of Spatiotemporal Dynamics of Active Nuclei DuringInternational journal of molecular sciences · 2025Article
- A conserved coupling of transcriptional ON and OFF periods underlies bursting dynamics.Nature structural & molecular biology · 2025Article
- Decoupling between activation time and steady-state level in input-output responses.bioRxiv : the preprint server for biology · 2025Article
- Bicoid-nucleosome competition sets a concentration threshold for transcription constrained by genome replication.Cell reports · 2025Article
- Novel Fluorescent and Photoconvertible Fusions Reveal Dorsal Activator Dynamics.bioRxiv : the preprint server for biology · 2025Article
- Deep learning-based high-resolution time inference for deciphering dynamic gene regulation from fixed embryos.Nature communications · 2025Article
- FGF and BMP pathway genes are expressed in segmentally iterated domains during segmentation of germ layers in a tardigrade.bioRxiv : the preprint server for biology · 2025Article
- Cell fate ratios are encoded by transcriptional dynamics in the Drosophila retina.Current biology : CB · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription.Development (Cambridge, England) · 2025Article
- MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans.The Journal of cell biology · 2025Article
- Deciphering HIV-1 Transcription Initiation and Elongation from Single-Molecule Imaging Data.Research (Washington, D.C.) · 2025Article
- A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in Drosophila.Development (Cambridge, England) · 2024Article
- Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription.bioRxiv : the preprint server for biology · 2024Article
- Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation.Development (Cambridge, England) · 2024Article
- Optogenetic dissection of transcriptional repression in a multicellular organism.Nature communications · 2024Article
46 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
The early Drosophila embryo is an ideal model to understand the transcriptional regulation of well-defined patterns of gene expression in a developing organism. In this system, snapshots of transcription measurements obtained by RNA FISH on fixed samples cannot provide the temporal resolution needed to distinguish spatial heterogeneity from inherent noise. Here, we used the MS2-MCP system to visualize in living embryos nascent transcripts expressed from the canonical hunchback (hb) promoter under the control of Bicoid (Bcd). The hb-MS2 reporter is expressed as synchronously as endogenous hb in the anterior half of the embryo, but unlike hb it is also active in the posterior, though more heterogeneously and more transiently than in the anterior. The length and intensity of active transcription periods in the anterior are strongly reduced in absence of Bcd, whereas posterior ones are mostly Bcd independent. This posterior noisy signal decreases progressively through nuclear divisions, so that the MS2 reporter expression mimics the known anterior hb pattern at cellularization. We propose that the establishment of the hb pattern relies on Bcd-dependent lengthening of transcriptional activity periods in the anterior and may require two distinct repression mechanisms in the posterior.
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