ArticleBiotechnology for biofuels2013
Adaptive evolution of an industrial strain of Saccharomyces cerevisiae for combined tolerance to inhibitors and temperature.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 155 citations in OpenAlex.
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- Short-term adaptation as a tool to improve bioethanol production using grass press-juice as fermentation medium.Applied microbiology and biotechnology · 2024Article
- Microfluidics for adaptation of microorganisms to stress: design and application.Applied microbiology and biotechnology · 2024Review
- Performance and robustness analysis reveals phenotypic trade-offs in yeast.Life science alliance · 2024Article
- A panoramic view of technological landscape for bioethanol production from various generations of feedstocks.Bioengineered · 2023Review
- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Strategies for the Development of Industrial Fungal Producing Strains.Journal of fungi (Basel, Switzerland) · 2023Review
- How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses.Current genetics · 2022Review
- Quantification of Microbial Robustness in Yeast.ACS synthetic biology · 2022Article
- Rational and evolutionary engineering of Saccharomyces cerevisiae for production of dicarboxylic acids from lignocellulosic biomass and exploring genetic mechanisms of the yeast tolerance to the biomass hydrolysate.Biotechnology for biofuels and bioproducts · 2022Article
- Transcriptomic analysis of formic acid stress response in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2022Article
- Metabolic changes of the acetogenFrontiers in microbiology · 2022Article
- Adaptive laboratory evolution of microbial co-cultures for improved metabolite secretion.Molecular systems biology · 2021Article
- Stress modulation as a means to improve yeasts for lignocellulose bioconversion.Applied microbiology and biotechnology · 2021Review
- A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species.Cell discovery · 2021Article
- Effects of Biosurfactants on Enzymatic Saccharification and Fermentation of Pretreated Softwood.Molecules (Basel, Switzerland) · 2020Article
- Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.Journal of applied microbiology · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDevelopment of industrial yeast strains with high tolerance towards the inhibitors released during biomass pretreatment is critical for bioethanol production. Combining this trait with increased thermotolerance would result in a more efficient production via Simultaneous Saccharification and Fermentation (SSF) as well as reduced cooling costs. The aim of the present work was to develop a yeast strain combining these traits.
resultsUsing a long-term adaptation strategy a stable Saccharomyces cerevisiae isolate (ISO12) was evolved from the industrial strain Ethanol Red (ER). ISO12, contrary to the parental strain, is capable of growing and fermenting the liquid fraction of non-detoxified spruce hydrolysate at 39°C with an ethanol yield of 0.38 g ethanol . g hexoses-1. In contrast with previous studies, the superior phenotype of ISO12 does not rely on higher reductase activities for furaldehyde inhibitor conversion, but rather on a higher thermotolerance. ISO12 shows a higher capacity to ferment hydrolysate at 39°C and higher viability during heat-shock at 52°C than ER. In the absence of inhibitors, however, both ER and ISO12 displayed similar growth phenotype at 39°C.
conclusionsThe evolved isolate ISO12 shows a superior phenotype than the parental strain ER when both stresses, temperature and inhibition by hydrolysate-derived compounds, are applied together. The results suggest that the presence of inhibitors depress the maximum temperature permissible for growth to a value below 39°C. As a result of the adaptation process and acquired improved thermotolerance, ISO12 is able to overcome this synergistic effect. Robust strains, such as ISO12, are interesting candidates for second generation ethanol production by SSF, as well as in tropical countries where fermentations at higher temperature can positively impact the production costs.
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