ArticleJournal of biological engineering2013
Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach.
Article in Journal of biological engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Coupling of biohydrogen and polyhydroxyalkanoates production processes for next-generation biorefineries: process design, optimization and perspectives.Bioprocess and biosystems engineering · 2026Review
- Molecular structural arrangement in quorum sensing and bacterial metabolic production.World journal of microbiology & biotechnology · 2025Review
- Valorization of Algal Biomass to Produce Microbial Polyhydroxyalkanoates: Recent Updates, Challenges, and Perspectives.Polymers · 2024Review
- Natural Polyhydroxyalkanoates-An Overview of Bacterial Production Methods.Molecules (Basel, Switzerland) · 2024Review
- N-terminal truncation of PhaCMicrobial cell factories · 2024Article
- Production of polyhydroxyalkanoates from renewable resources: a review on prospects, challenges and applications.Archives of microbiology · 2023Review
- Microalgae in Bioplastic Production: A Comprehensive Review.Arabian journal for science and engineering · 2023Review
- The transcription activator AtxA fromSynthetic biology (Oxford, England) · 2022Article
- Biological Approaches in Polyhydroxyalkanoates Recovery.Current microbiology · 2021Review
- Microbial-Derived Polyhydroxyalkanoate-Based Scaffolds for Bone Tissue Engineering: Biosynthesis, Properties, and Perspectives.Frontiers in bioengineering and biotechnology · 2021Review
- Challenges and Perspectives of Polyhydroxyalkanoate Production From Microalgae/Cyanobacteria and Bacteria as Microbial Factories: An Assessment of Hybrid Biological System.Frontiers in bioengineering and biotechnology · 2021Review
- Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- Reconfiguring Plant Metabolism for Biodegradable Plastic Production.Biodesign research · 2020Review
- Prospects for the Use of Whey for Polyhydroxyalkanoate (PHA) Production.Frontiers in microbiology · 2019Review
- Food waste conversion to microbial polyhydroxyalkanoates.Microbial biotechnology · 2017Review
- Expression of the entire polyhydroxybutyrate operon ofJournal of biological engineering · 2017Article
- Microbubble assisted polyhydroxybutyrate production in Escherichia coli.BMC research notes · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolyhydroxyalkanoates (PHAs) are a group of biodegradable plastics that are produced by a wide variety of microorganisms, mainly as a storage intermediate for energy and carbon. Polyhydroxybutyrate (PHB) is a short-chain-length PHA with interesting chemical and physical properties. Large scale production of PHB is not wide-spread mainly due to the downstream processing of bacterial cultures to extract the PHB. Secretion of PHB from Escherichia coli could reduce downstream processing costs. PHB are non-proteinaceous polymers, hence cannot be directly targeted for secretion. Phasin, PhaP1, is a low molecular weight protein that binds to PHB, reducing PHB granule size. In this study PHB is indirectly secreted with PhaP1 from E. coli via type I secretion using HlyA signal peptides.
resultsThis study demonstrated the successful secretion of phasin and phasin bound PHB outside of the cell and into the culture medium. The secretion of PHB was initiated between 24 and 48 h after induction. After 48 h of culturing, 36% of the total PHB produced in the secreting strain was collected in the secreted fraction and 64% remained in the internal fraction. To further support the findings of this study, the PHB secretion phenomenon was observed using SEM.
conclusionsFrom this study, the ability to use type I secretion to: 1) secrete phasin and 2) successfully secrete PHB has been shown.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.