ArticleBiophysical journal2013
IR laser-induced perturbations of the voltage-dependent solute carrier protein SLC26a5.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Optical induction of auditory perception via cochlear stimulation in Mongolian gerbils without genetic modification.iScience · 2026Article
- Fast and serious: A new method to study ion channel thermodynamics.Biophysical journal · 2023Article
- Ion channel thermodynamics studied with temperature jumps measured at the cell membrane.Biophysical journal · 2023Article
- Coupling between outer hair cell electromotility and prestin sensor charge depends on voltage operating point.Hearing research · 2022Article
- Bidirectional modulation of evoked synaptic transmission by pulsed infrared light.Scientific reports · 2022Article
- State dependent effects on the frequency response of prestin's real and imaginary components of nonlinear capacitance.Scientific reports · 2021Article
- Infrared neural stimulation at different wavelengths and pulse shapes.Progress in biophysics and molecular biology · 2021Article
- Near-infrared stimulation of the auditory nerve: A decade of progress toward an optical cochlear implant.Laryngoscope investigative otolaryngology · 2021Review
- The cochlear outer hair cell speed paradox.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Complex nonlinear capacitance in outer hair cell macro-patches: effects of membrane tension.Scientific reports · 2020Article
- Prestin kinetics and corresponding frequency dependence augment during early development of the outer hair cell within the mouse organ of Corti.Scientific reports · 2019Article
- Multichannel optrodes for photonic stimulation.Neurophotonics · 2018Article
- Pressure in the Cochlea During Infrared Irradiation.IEEE transactions on bio-medical engineering · 2018Article
- The Frequency Response of Outer Hair Cell Voltage-Dependent Motility Is Limited by Kinetics of Prestin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Auditory Neural Activity in Congenitally Deaf Mice Induced by Infrared Neural Stimulation.Scientific reports · 2018Article
- Bringing new dimensions to drug discovery screening: impact of cellular stimulation technologies.Drug discovery today · 2017Review
- Neural Crest Stem Cells Can Differentiate to a Cardiomyogenic Lineage with an Ability to Contract in Response to Pulsed Infrared Stimulation.Tissue engineering. Part C, Methods · 2016Article
- Heat pulse excitability of vestibular hair cells and afferent neurons.Journal of neurophysiology · 2016Article
- Chloride Anions Regulate Kinetics but Not Voltage-Sensor Qmax of the Solute Carrier SLC26a5.Biophysical journal · 2016Article
- Analysis of the Damage Mechanism Related to CONeural plasticity · 2016Article
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2 authors.
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Abstract
Alterations in membrane capacitance can arise from linear and nonlinear sources. For example, changes in membrane surface area or dielectric properties can modify capacitance linearly, whereas sensor residues of voltage-dependent proteins can modify capacitance nonlinearly. Here, we examined the effects of fast temperature jumps induced by an infrared (IR) laser in control and prestin (SLC26a5)-transfected human embryonic kidney (HEK) cells under whole-cell voltage clamp. Prestin's voltage sensor imparts a characteristic bell-shaped, voltage-dependent nonlinear capacitance (NLC). Temperature jumps in control HEK cells cause a monophasic increase in membrane capacitance (Cm) regardless of holding voltage due to double-layer effects. Prestin-transfected HEK cells, however, additionally show a biphasic increase/decrease in Cm with a reversal potential corresponding to the voltage at peak NLC of prestin (Vh), attributable to a rapid temperature-following shift in Vh, with shift rates up to 14 V/s over the course of a 5 ms IR pulse. Treatment with salicylate, a known inhibitor of NLC, reestablishes control cell behavior. A simple kinetic model recapitulates our biophysical observations. These results verify a voltage-dependent protein's ability to respond to fast temperature perturbations on a par with double-layer susceptibility. This likely arises from prestin's unique ability to move sensor charge at kilohertz rates, which is required for the outer hair cells' role as a cochlear amplifier.
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