ArticleBiophysical journal2013
Electronic transitions and heterogeneity of the bacteriophytochrome Pr absorption band: An angle balanced polarization resolved femtosecond VIS pump-IR probe study.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Ultrafast, remote-controlled protonation reaction enables structural changes in a phytochrome.Science advances · 2025Article
- Integrated Study of Fluorescence Enhancement in the Y176H Variant of Cyanobacterial Phytochrome Cph1.Biochemistry · 2025Article
- Vibrational Spectroscopy of Phytochromes.Biomolecules · 2023Review
- Ultrafast protein response in the Pfr state of Cph1 phytochrome.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Ultrafast Photoconversion Dynamics of the Knotless PhytochromeInternational journal of molecular sciences · 2021Article
- The Origin of Ultrafast Multiphasic Dynamics in Photoisomerization of Bacteriophytochrome.The journal of physical chemistry letters · 2020Article
- The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Blue protein with red fluorescence.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ultrafast electronic and vibrational dynamics in brominated aluminum corroles: Energy relaxation and triplet formation.Structural dynamics (Melville, N.Y.) · 2016Article
- Femtosecond infrared spectroscopy of channelrhodopsin-1 chromophore isomerization.Structural dynamics (Melville, N.Y.) · 2016Article
- Fast Photochemistry of Prototypical Phytochromes-A Species vs. Subunit Specific Comparison.Frontiers in molecular biosciences · 2015Article
- Conformational heterogeneity of the Pfr chromophore in plant and cyanobacterial phytochromes.Frontiers in molecular biosciences · 2015Article
- Comprehensive analysis of the green-to-blue photoconversion of full-length Cyanobacteriochrome Tlr0924.Biophysical journal · 2014Article
- Active and silent chromophore isoforms for phytochrome Pr photoisomerization: An alternative evolutionary strategy to optimize photoreaction quantum yields.Structural dynamics (Melville, N.Y.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoisomerization of biliverdin (BV) chromophore triggers the photoresponse in native Agp1 bacteriophytochrome. We discuss heterogeneity in phytochrome Pr form to account for the shape of the absorption profile. We investigated different regions of the absorption profile by angle balanced polarization resolved femtosecond VIS pump-IR probe spectroscopy. We studied the Pr form of Agp1 with its natural chromophore and with a sterically locked 18Et-BV (locked Agp1). We followed the dynamics and orientations of the carbonyl stretching vibrations of ring D and ring A in their ground and electronically excited states. Photoisomerization of ring D is reflected by strong signals of the ring D carbonyl vibration. In contrast, orientational data on ring A show no rotation of ring A upon photoexcitation. Orientational data allow excluding a ZZZasa geometry and corroborates a nontwisted ZZZssa geometry of the chromophore. We found no proof for heterogeneity but identified a new, to our knowledge, electronic transition in the absorption profile at 644 nm (S0→S2). Excitation of the S0→S2 transition will introduce a more complex photodynamics compared with S0→S1 transition. Our approach provides fundamental information on disentanglement of absorption profiles, identification of chromophore structures, and determination of molecular groups involved in the photoisomerization process of photoreceptors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.