ArticleNucleic acids research2014
High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 97 citations in OpenAlex.
- The shared evolutionary capacities of plasmids and extrachromosomal DNA.Nature reviews. Genetics · 2026Review
- Large extrachromosomal replicons are widespread across bacterial lineages and show coordinated replication termination and spatial coupling with the chromosome.Nature communications · 2026Article
- Review
- ProFiT-SPEci-FISH: a novel approach for linking plasmids to hosts in complex microbial communities at the single-cell level.Microbiome · 2025Article
- Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states.Biochemistry and biophysics reports · 2025Article
- Inhibiting Amikacin Resistance in Multidrug-Resistant Bacteria with Cadmium and Pyrithione.Current microbiology · 2025Review
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- Visualization of Type IV-A1 CRISPR-mediated repression of gene expression and plasmid replication.Nucleic acids research · 2024Article
- Spatial mapping of mobile genetic elements and their bacterial hosts in complex microbiomes.Nature microbiology · 2024Article
- Dynamic structure of E. coli cytoplasm: supramolecular complexes and cell aging impact spatial distribution and mobility of proteins.Communications biology · 2024Article
- Article
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Role of Xer site-specific recombination in the genesis of pJHCMW1: an evolutionary hypothesis.Journal of global antimicrobial resistance · 2023Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Article
- Spatial Mapping of Mobile Genetic Elements and their Cognate Hosts in Complex Microbiomes.bioRxiv : the preprint server for biology · 2023Article
- The parABSm system is involved in megaplasmid partitioning and genome integrity maintenance in Thermus thermophilus.G3 (Bethesda, Md.) · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacterial plasmids play important roles in the metabolism, pathogenesis and bacterial evolution and are highly versatile biotechnological tools. Stable inheritance of plasmids depends on their autonomous replication and efficient partition to daughter cells at cell division. Active partition systems have not been identified for high-copy number plasmids, and it has been generally believed that they are partitioned randomly at cell division. Nevertheless, direct evidence for the cellular location of replicating and nonreplicating plasmids, and the partition mechanism has been lacking. We used as model pJHCMW1, a plasmid isolated from Klebsiella pneumoniae that includes two β-lactamase and two aminoglycoside resistance genes. Here we report that individual ColE1-type plasmid molecules are mobile and tend to be excluded from the nucleoid, mainly localizing at the cell poles but occasionally moving between poles along the long axis of the cell. As a consequence, at the moment of cell division, most plasmid molecules are located at the poles, resulting in efficient random partition to the daughter cells. Complete replication of individual molecules occurred stochastically and independently in the nucleoid-free space throughout the cell cycle, with a constant probability of initiation per plasmid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.