ArticleNucleic acids research2014
Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- A selective antibiotic for Lyme disease.Cell · 2021Article
- Structural basis of l-tryptophan-dependent inhibition of release factor 2 by the TnaC arrest peptide.Nucleic acids research · 2021Article
- Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling.Nature communications · 2021Article
- Conserved Upstream Open Reading Frame Nascent Peptides That Control Translation.Annual review of genetics · 2020Review
- A fully orthogonal system for protein synthesis in bacterial cells.Nature communications · 2020Article
- Dynamics of transcription-translation coordination tune bacterial indole signaling.Nature chemical biology · 2020Article
- The regulatory TnaC nascent peptide preferentially inhibits release factor 2-mediated hydrolysis of peptidyl-tRNA.The Journal of biological chemistry · 2019Article
- Regulation of Bacterial Gene Expression by Transcription Attenuation.Microbiology and molecular biology reviews : MMBR · 2019Review
- Article
- How Macrolide Antibiotics Work.Trends in biochemical sciences · 2018Review
- Rebuilding the bridge between transcription and translation.Molecular microbiology · 2018Article
- Identification of Arabidopsis thaliana upstream open reading frames encoding peptide sequences that cause ribosomal arrest.Nucleic acids research · 2017Article
- Learning from the Leaders: Gene Regulation by the Transcription Termination Factor Rho.Trends in biochemical sciences · 2016Review
- Regulation of bacterial gene expression by ribosome stalling and rescuing.Current genetics · 2016Review
- Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats.RNA (New York, N.Y.) · 2015Article
- Identification of novel Arabidopsis thaliana upstream open reading frames that control expression of the main coding sequences in a peptide sequence-dependent manner.Nucleic acids research · 2015Article
- Ribosomes left in the dust: diverse strategies for Peptide-mediated translation stalling.Molecular cell · 2014Article
- Translational stalling at polyproline stretches is modulated by the sequence context upstream of the stall site.Nucleic acids research · 2014Article
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
A transcriptional attenuation mechanism regulates expression of the bacterial tnaCAB operon. This mechanism requires ribosomal arrest induced by the regulatory nascent TnaC peptide in response to free L-tryptophan (L-Trp). In this study we demonstrate, using genetic and biochemical analyses, that in Escherichia coli, TnaC residue I19 and 23S rRNA nucleotide A2058 are essential for the ribosome's ability to sense free L-Trp. We show that the mutational change A2058U in 23S rRNA reduces the concentration dependence of L-Trp-mediated tna operon induction, whereas the TnaC I19L change suppresses this phenotype, restoring the sensitivity of the translating A2058U mutant ribosome to free L-Trp. These findings suggest that interactions between TnaC residue I19 and 23S rRNA nucleotide A2058 contribute to the creation of a regulatory L-Trp binding site within the translating ribosome.
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