Evidence map›Paper›PMID 24136211›Full record

ArticleJournal of veterinary science2014

Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil.

Rosiane Nascimento Alves, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo EmÍlio Beletti

Open access · goldAbstract read
In one paragraph

Article in Journal of veterinary science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Molecular Investigation of the p28 Gene ofVeterinary medicine international · 2025
    Article
  2. Molecular characterization of Ehrlichia canis from naturally infected dogs from the state of Rio de Janeiro.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Rosiane Nascimento AlvesInstitute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405-320, Brazil.rnalves@icbim.ufu.br.
Susana Elisa Rieck
Carlos Ueira-Vieira
Marcelo Bahia Labruna
Marcelo EmÍlio Beletti
Universidade Federal de Uberlândia · BRInstituto Federal de Educação, Ciência e Tecnologia do Triângulo Mineiro · BRUniversidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlandia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlândia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlândia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlândia and São Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.

Indexed as

Antigens, BacterialAnimalsBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBrazilDog DiseasesDogsEhrlichia canisEhrlichiosisFluorescent Antibody Technique, IndirectMaleMolecular Sequence DataPolymerase Chain ReactionRNA, Ribosomal, 16SSequence AlignmentAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsRNA, Ribosomal, 16SBrazilEhrlichia canisgenetic characterizationisolationserology

Identifiers

PMID24136211
PMCPMC4087226
OpenAlexW2090831845

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.