ArticleMolecular neurobiology2014
Expression stability of reference genes for quantitative RT-PCR of healthy and diseased pituitary tissue samples varies between humans, mice, and dogs.
Article in Molecular neurobiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Postmortem tissue biomarkers of menopausal transition.Molecular psychiatry · 2026Article
- Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR.BMC neuroscience · 2025Article
- Evaluation of Reference Gene Stability in Goat Skeletal Muscle Satellite Cells during Proliferation and Differentiation Phases.Animals : an open access journal from MDPI · 2024Article
- PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic-pituitary-gonadal axis in sows.Animal bioscience · 2022Article
- Transcriptome-based selection and validation of optimal reference genes in perirenal adipose developing of goat (Frontiers in veterinary science · 2022Article
- Potential biomarkers and lncRNA-mRNA regulatory networks in invasive growth hormone-secreting pituitary adenomas.Journal of endocrinological investigation · 2021Article
- Butyrate mediates anti-inflammatory effects ofGut microbes · 2020Article
- High-Fat Diet Alters the Expression of Reference Genes in Male Mice.Frontiers in nutrition · 2020Article
- Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.Scientific reports · 2019Article
- Article
- Development and application of a canine endogenous internal positive control for use in real-time PCR assays.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- New Insights in the Functional Zonation of the Canine Adrenal Cortex.Journal of veterinary internal medicine · 2016Article
- Validating candidate reference genes for qRT-PCR-based gene expression analysis in nonfunctioning pituitary adenomas.Pituitary · 2016Article
- Positive expression of KIF20A indicates poor prognosis of glioma patients.OncoTargets and therapy · 2016Article
- Reference genes for reverse transcription quantitative PCR in canine brain tissue.BMC research notes · 2015Article
- Laccase Production and Differential Transcription of Laccase Genes in Cerrena sp. in Response to Metal Ions, Aromatic Compounds, and Nutrients.Frontiers in microbiology · 2015Article
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Authors and funding
7 authors.
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Abstract
Pituitary surgery generates pituitary tissue for histology, immunohistochemistry, and molecular biological research. In the last decade, the pathogenesis of pituitary adenomas has been extensively studied in humans, and to a lesser degree in dogs, and tumor oncogenesis has been studied in knock-out mice, often by means of quantitative reversed-transcriptase PCR (RT-qPCR). A precondition of such analyses is that so-called reference genes are stably expressed regardless of changes in disease status or treatment. In this study, the expression of six frequently used reference genes, namely, tata box binding protein (tbp), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (ywhaz), hydroxymethylbilane synthase (hmbs), beta-2-microglobulin (b2m), succinate dehydrogenase complex subunit A (sdha), and glyceraldehyde 3 phosphate dehydrogenase 1 (gapdh), was studied in pituitary tissue (normal and adenoma) from three species (humans, mice, and dogs). The stability of expression of these reference genes differed between species and between healthy and diseased tissue within one species. Quantitative analysis based on a single reference gene that is assumed to be stably expressed might lead to wrong conclusions. This cross-species analysis clearly emphasizes the need to evaluate the expression stability of reference genes as a standard and integral aspect of study design and data analysis, in order to improve the validity of the conclusions drawn on the basis of quantitative molecular analyses.
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