ArticleFly2014
Bi-functional cross-linking reagents efficiently capture protein-DNA complexes in Drosophila embryos.
Article in Fly, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Chromatin Immunoprecipitation Sequencing in Cancer Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Identification and partial characterization of new cell density-dependent nucleocytoplasmic shuttling proteins and open chromatin.Scientific reports · 2023Article
- Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription inInternational journal of molecular sciences · 2021Article
- Photocaged Quinone Methide Crosslinkers for Light-Controlled Chemical Crosslinking of Protein-Protein and Protein-DNA Complexes.Angewandte Chemie (International ed. in English) · 2019Article
- BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila.Nature communications · 2019Article
- Article
- Optimized ChIP-seq method facilitates transcription factor profiling in human tumors.Life science alliance · 2019Article
- Functional dissection of the developmentally restricted BEN domain chromatin boundary factor Insensitive.Epigenetics & chromatin · 2019Article
- The BEN Domain Protein Insensitive Binds to theGenetics · 2018Article
- Streamlined discovery of cross-linked chromatin complexes and associated histone modifications by mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Chromatin immunoprecipitation (ChIP) is widely used for mapping DNA-protein interactions across eukaryotic genomes in cells, tissues or even whole organisms. Critical to this procedure is the efficient cross-linking of chromatin-associated proteins to DNA sequences that are in close proximity. Since the mid-nineties formaldehyde fixation has been the method of choice. However, some protein-DNA complexes cannot be successfully captured for ChIP using formaldehyde. One such formaldehyde refractory complex is the developmentally regulated insulator factor, Elba. Here we describe a new embryo fixation procedure using the bi-functional cross-linking reagents DSG (disuccinimidyl glutarate) and DSP (dithiobis[succinimidyl propionate). We show that unlike standard formaldehyde fixation protocols, it is possible to capture Elba association with insulator elements in 2-5 h embryos using this new cross-linking procedure. We show that this new cross-linking procedure can also be applied to localize nuclear proteins that are amenable to ChIP using standard formaldehyde cross-linking protocols, and that in the cases tested the enrichment was generally superior to that achieved using formaldehyde cross-linking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.