ArticleJournal of structural biology2013
3-D analysis of dictyosomes and multivesicular bodies in the green alga Micrasterias denticulata by FIB/SEM tomography.
Article in Journal of structural biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exosome Biogenesis: Meta-Analysis of Intraluminal Vesicle Size Across Species.International journal of molecular sciences · 2026Pooled it
- Three-dimensional analysis of membrane structures associated with tomato spotted wilt virus infection.Plant, cell & environment · 2023Article
- The cell biology of charophytes: Exploring the past and models for the future.Plant physiology · 2022Article
- Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning.Planta · 2022Article
- Changes in Envelope Structure and Cell-Cell Communication during Akinete Differentiation and Germination in Filamentous CyanobacteriumLife (Basel, Switzerland) · 2022Article
- Winter survival of the unicellular green alga Micrasterias denticulata: insights from field monitoring and simulation experiments.Protoplasma · 2021Article
- 3D Dissection of Structural Membrane-Wall Contacts in Filamentous Moss Protonemata.International journal of molecular sciences · 2020Article
- Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants.International journal of molecular sciences · 2020Article
- Cell Wall Reinforcements Accompany Chilling and Freezing Stress in the Streptophyte Green AlgaFrontiers in plant science · 2020Article
- Quantifying Golgi structure using EM: combining volume-SEM and stereology for higher throughput.Histochemistry and cell biology · 2017Review
- Charophytes: Evolutionary Giants and Emerging Model Organisms.Frontiers in plant science · 2016Review
- Micrasterias as a Model System in Plant Cell Biology.Frontiers in plant science · 2016Review
- Abiotic Stress Tolerance of Charophyte Green Algae: New Challenges for Omics Techniques.Frontiers in plant science · 2016Review
- Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.eLife · 2014Article
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3 authors.
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Abstract
In the present study we employ FIB/SEM tomography for analyzing 3-D architecture of dictyosomes and formation of multivesicular bodies (MVB) in high pressure frozen and cryo-substituted interphase cells of the green algal model system Micrasterias denticulata. The ability of FIB/SEM of milling very thin 'slices' (5-10 nm), viewing the block face and of capturing cytoplasmic volumes of several hundred μm(3) provides new insight into the close spatial connection of the ER-Golgi machinery in an algal cell particularly in z-direction, complementary to informations obtained by TEM serial sectioning or electron tomography. Our FIB/SEM series and 3-D reconstructions show that interphase dictyosomes of Micrasterias are not only closely associated to an ER system at their cis-side which is common in various plant cells, but are surrounded by a huge "trans-ER" sheath leading to an almost complete enwrapping of dictyosomes by the ER. This is particularly interesting as the presence of a trans-dictyosomal ER system is well known from mammalian secretory cells but not from cells of higher plants to which the alga Micrasterias is closely related. In contrast to findings in plant storage tissue indicating that MVBs originate from the trans-Golgi network or its derivatives our investigations show that MVBs in Micrasterias are in direct spatial contact with both, trans-Golgi cisternae and the trans-ER sheath which provides evidence that both endomembrane compartments are involved in their formation.
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